ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKNKGKGGK NRKRGKNEAD DEKRELIFKE DGQEYAQVLR MLGNGRCDVM CIDGVKRLCH 

        70         80         90        100        110        120 
IRGKMHKKVW IAAGDIILVG LRDYQDDKAD VILKYMSDEA RLLKAYGELP ENTRLNEGIV 

       130        140 
GDLDDDDDVV GEDYIEFEDE DIDRI 

References and documentation are available.

Number of amino acids: 145

Molecular weight: 16589.78

Theoretical pI: 4.89

Amino acid composition: 
Ala (A)   7	  4.8%
Arg (R)  11	  7.6%
Asn (N)   6	  4.1%
Asp (D)  21	 14.5%
Cys (C)   3	  2.1%
Gln (Q)   3	  2.1%
Glu (E)  13	  9.0%
Gly (G)  15	 10.3%
His (H)   2	  1.4%
Ile (I)  11	  7.6%
Leu (L)  12	  8.3%
Lys (K)  15	 10.3%
Met (M)   5	  3.4%
Phe (F)   2	  1.4%
Pro (P)   2	  1.4%
Ser (S)   1	  0.7%
Thr (T)   1	  0.7%
Trp (W)   1	  0.7%
Tyr (Y)   5	  3.4%
Val (V)   9	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	       715
Hydrogen    H	      1157
Nitrogen    N	       207
Oxygen      O	       230
Sulfur      S	         8

Formula: C715H1157N207O230S8
Total number of atoms: 2317

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13075
Abs 0.1% (=1 g/l)   0.788, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.781, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.93
This classifies the protein as unstable.



Aliphatic index: 84.69

Grand average of hydropathicity (GRAVY): -0.792