ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKRFSKKVA IITGSSSGIG RETALLFAKE GAKVTVTGRS EEKLEETKKA LLDAGIKESN 

        70         80         90        100        110        120 
FLIVPADITF STGQDELISQ TLKKFGRINI LVNNAGASIP DAKKRTGIDQ GIETYEQVMK 

       130        140        150        160        170        180 
LNVQSVIEMT QKVRPHLAKS RGEIVNVSSV VALKAGWTRT PYYPLAKAAL DQYTRSAAIA 

       190        200        210        220        230        240 
LISEGIRVNT VNPGIVQTGF QANASGSSSD DAQKFYDDMG SNTTAIPCGF AGRPEHIAKA 

       250        260        270 
IAFLADRNSS EYIVGQNIIA DGGTSLVLGI HGPAGGKK 

References and documentation are available.

Number of amino acids: 278

Molecular weight: 29555.73

Theoretical pI: 9.37

Amino acid composition: 
Ala (A)  30	 10.8%
Arg (R)  12	  4.3%
Asn (N)  12	  4.3%
Asp (D)  12	  4.3%
Cys (C)   1	  0.4%
Gln (Q)  11	  4.0%
Glu (E)  15	  5.4%
Gly (G)  27	  9.7%
His (H)   3	  1.1%
Ile (I)  25	  9.0%
Leu (L)  18	  6.5%
Lys (K)  22	  7.9%
Met (M)   4	  1.4%
Phe (F)   9	  3.2%
Pro (P)  10	  3.6%
Ser (S)  23	  8.3%
Thr (T)  19	  6.8%
Trp (W)   1	  0.4%
Tyr (Y)   6	  2.2%
Val (V)  18	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1304
Hydrogen    H	      2126
Nitrogen    N	       366
Oxygen      O	       404
Sulfur      S	         5

Formula: C1304H2126N366O404S5
Total number of atoms: 4205

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.489, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.489, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.71
This classifies the protein as stable.



Aliphatic index: 89.89

Grand average of hydropathicity (GRAVY): -0.165