ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKRIVYNIS SDFQLKSLLG EGAYGVVCSA THKPTGEIVA IKKIEPFDKP LFALRTLREI 

        70         80         90        100        110        120 
KILKHFKHEN IITIFNIQRP DSFENFNEVY IIQELMQTDL HRVISTQMLS DDHIQYFIYQ 

       130        140        150        160        170        180 
TLRAVKVLHG SNVIHRDLKP SNLLINSNCD LKVCDFGLAR IIDESAADNS EPTGQQSGMT 

       190        200        210        220        230        240 
EYVATRWYRA PEVMLTSAKY SRAMDVWSCG CILAELFLRR PIFPGRDYRH QLLLIFGIIG 

       250        260        270        280        290        300 
TPHSDNDLRC IESPRAREYI KSLPMYPAAP LEKMFPRVNP KGIDLLQRML VFDPAKRITA 

       310        320        330        340        350 
KEALEHPYLQ TYHDPNDEPE GEPIPPSFFE FDHYKEALTT KDLKKLIWNE IFS 

References and documentation are available.

Number of amino acids: 353

Molecular weight: 40772.07

Theoretical pI: 6.64

Amino acid composition: 
Ala (A)  20	  5.7%
Arg (R)  21	  5.9%
Asn (N)  14	  4.0%
Asp (D)  21	  5.9%
Cys (C)   6	  1.7%
Gln (Q)  12	  3.4%
Glu (E)  24	  6.8%
Gly (G)  14	  4.0%
His (H)  12	  3.4%
Ile (I)  32	  9.1%
Leu (L)  35	  9.9%
Lys (K)  22	  6.2%
Met (M)   9	  2.5%
Phe (F)  18	  5.1%
Pro (P)  24	  6.8%
Ser (S)  21	  5.9%
Thr (T)  16	  4.5%
Trp (W)   3	  0.8%
Tyr (Y)  14	  4.0%
Val (V)  15	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1846
Hydrogen    H	      2883
Nitrogen    N	       491
Oxygen      O	       521
Sulfur      S	        15

Formula: C1846H2883N491O521S15
Total number of atoms: 5756

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37735
Abs 0.1% (=1 g/l)   0.926, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   0.916, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.51
This classifies the protein as unstable.



Aliphatic index: 92.01

Grand average of hydropathicity (GRAVY): -0.288