ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKRKVTFQG VGDEEDEDEI IVPKKKLVDP VAGSGGPGSR FKGKHSLDSD EEEDDDDGGS 

        70         80         90        100        110        120 
SKYDILASED VEGQEAATLP SEGGVRITPF NLQEEMEEGH FDADGNYFLN RDAQIRDSWL 

       130        140        150        160        170        180 
DNIDWVKIRE RPPGQRQASD SEEEDSLGQT SMSAQALLEG LLELLLPRET VAGALRRLGA 

       190        200        210        220        230        240 
RGGGKGRKGP GQPSSPQRLD RLSGLADQMV ARGNLGVYQE TRERLAMRLK GLGCQTLGPH 

       250        260        270        280        290        300 
NPTPPPSLDM FAEELAEEEL ETPTPTQRGE AESRGDGLVD VMWEYKWENT GDAELYGPFT 

       310        320        330        340 
SAQMQTWVSE GYFPDGVYCR KLDPPGGQFY NSKRIDFDLY T 

References and documentation are available.

Number of amino acids: 341

Molecular weight: 37646.46

Theoretical pI: 4.49

Amino acid composition: 
Ala (A)  20	  5.9%
Arg (R)  23	  6.7%
Asn (N)   8	  2.3%
Asp (D)  30	  8.8%
Cys (C)   2	  0.6%
Gln (Q)  17	  5.0%
Glu (E)  36	 10.6%
Gly (G)  40	 11.7%
His (H)   3	  0.9%
Ile (I)   8	  2.3%
Leu (L)  32	  9.4%
Lys (K)  15	  4.4%
Met (M)   8	  2.3%
Phe (F)  10	  2.9%
Pro (P)  23	  6.7%
Ser (S)  22	  6.5%
Thr (T)  15	  4.4%
Trp (W)   5	  1.5%
Tyr (Y)   9	  2.6%
Val (V)  15	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1631
Hydrogen    H	      2539
Nitrogen    N	       461
Oxygen      O	       545
Sulfur      S	        10

Formula: C1631H2539N461O545S10
Total number of atoms: 5186

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41035
Abs 0.1% (=1 g/l)   1.090, assuming all pairs of Cys residues form cystines


Ext. coefficient    40910
Abs 0.1% (=1 g/l)   1.087, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.31
This classifies the protein as unstable.



Aliphatic index: 64.37

Grand average of hydropathicity (GRAVY): -0.828