ProtParam
User-provided sequence:
10 20 30 40 50 60
MPKSKRDRPV TLSKTKKKGR EHKECIVNGI REAVEKYSSV YVFSFENMRN IKFKEFRQQF
70 80 90 100 110 120
RHNGKFFLGS NKVMQVALGR SAEDELRSGI YKVSKLLRGD TGLLVTDMPK EEVESLFNAY
130 140 150 160 170 180
EDSDFSRTGS IAVETVELKE GPLEQFTHEM EPLLRKLEMP VRLNKGTVEL VADFVVCEEG
190 200 210 220 230
KQLSPKSAHI LRLLRMKMAT FKLNLLCRWS PSDFELYRED LSELYREDLS DVETS
References and
documentation are available.
Number of amino acids: 235
Molecular weight: 27242.33
Theoretical pI: 8.31
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1207
Hydrogen H 1937
Nitrogen N 333
Oxygen O 361
Sulfur S 11
Formula: C
1207H
1937N
333O
361S
11
Total number of atoms: 3849
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14565
Abs 0.1% (=1 g/l) 0.535, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.530, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.49
This classifies the protein as unstable.
Aliphatic index: 80.38
Grand average of hydropathicity (GRAVY): -0.548