ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPKVTKEKKS RIHNDVQKQG IVFNKDFGQH ILKNPLVITT MLEKAALRAT DVVLEIGPGT 

        70         80         90        100        110        120 
GNMTVRMLER AKKVIACEID TRLAAELQKR VQATPLQPKL QVLIGDFLKA ELPFFDLCIA 

       130        140        150        160        170        180 
NVPYQISSPL IFKLLLHRPL FRCAVLMFQR EFAERLVAKP GDKLYCRLSI NTQLLARVDM 

       190        200        210        220        230        240 
LMKVGKNNFR PPPKVESSVV RLEPKNPPPP VNFTEWDGLT RIAFLRKNKT LAATFKVTSV 

       250        260        270        280        290        300 
LEMLEKNYKL YRSLRNEPIE DDFKMQDKVI SILEEQDMAA KRARSMDIDD FMRLLLAFNS 


AGIHFN 

References and documentation are available.

Number of amino acids: 306

Molecular weight: 35048.44

Theoretical pI: 9.69

Amino acid composition: 
Ala (A)  22	  7.2%
Arg (R)  21	  6.9%
Asn (N)  15	  4.9%
Asp (D)  16	  5.2%
Cys (C)   4	  1.3%
Gln (Q)  12	  3.9%
Glu (E)  18	  5.9%
Gly (G)  10	  3.3%
His (H)   4	  1.3%
Ile (I)  18	  5.9%
Leu (L)  38	 12.4%
Lys (K)  28	  9.2%
Met (M)  12	  3.9%
Phe (F)  17	  5.6%
Pro (P)  19	  6.2%
Ser (S)  11	  3.6%
Thr (T)  14	  4.6%
Trp (W)   1	  0.3%
Tyr (Y)   4	  1.3%
Val (V)  22	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      1580
Hydrogen    H	      2577
Nitrogen    N	       433
Oxygen      O	       431
Sulfur      S	        16

Formula: C1580H2577N433O431S16
Total number of atoms: 5037

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11710
Abs 0.1% (=1 g/l)   0.334, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.327, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.62
This classifies the protein as stable.



Aliphatic index: 99.41

Grand average of hydropathicity (GRAVY): -0.168