ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPLAFTNPGT PLKRTAAYDN VQWNQNRILT FGTRYTVLII VNNYESYQNI QFGESIGAVA 

        70         80         90        100        110        120 
LDQRTGFLAI AHGQVLDIYK PADSLKWTHH YNYITQLSED ITQLSWGYNF DLLLCASSYT 

       130        140        150        160        170        180 
KLINFSEDKP RLVWSQNRIS DSFLSSTISS DACFIASIET KNPLAKVWQR TSPQGTVSSC 

       190        200        210        220        230        240 
DDFSYLVHPC RVRFSQWSKH LHPDASGSTP FLSTVGYDNV LRIWQVGSRF GSQLMHLSCF 

       250        260        270        280        290        300 
LNLRKYYKSD QPPYCCFIDK DDFTIALAHA ISRHSGQVHK DRILNRLLAL ASGQPHIFLL 

       310        320        330        340        350        360 
FTFECLYIFS LVIDQETLSS FELINQVPTN FPKGALQPDG FIPIYRTSKH EFYDYNIILF 

       370        380        390        400        410        420 
FQNSAREYMF SLTDFFYSDF ELRVSYDFYG HDYPIKSLTR TSNGHGIVSR DVNRICMFQS 

       430        440        450        460        470        480 
YISQDGKNQL ICRFRLLLGE SDFILPLYAG EYAAVLNLNS LKLWHCNEAI PPTLLCTCRQ 

       490        500        510        520        530        540 
VPTSPIITFF ILPVQNNDNS ALCALTEGGH AWFWSVSNEA HDGSAVLRFI DRVNFTKNLN 

       550        560        570        580        590        600 
IASAVDVMGW SSTLNLSSLS SFDQEVFQSI SKDGLLQTWM ARVLGDDKAE ISEISKVQTS 

       610        620        630        640        650        660 
IKSATMIKGS TSKKVAVVSE NNRRLSIFDT RSSEFSEKEE FSSVFDDYGP INDLDWTSTP 

       670        680        690        700        710        720 
NSHSILAVGF LHDVILLCQN RRSYMNDIPC WSRIRRYNLL EYTNSLISDS SWLDNGTLVT 

       730        740        750        760        770        780 
AIGNGLFYFD NSLPEDQSLL FPASLKRSAK NIFDLAYQLN GILPVFHPQL LQQLLLRNQP 

       790        800        810        820        830        840 
FLFTNILLRF YLCLKDDVDM HFLLNMDCSE FYCCDEEISK DLLIKSLSQN SAEILNPAEL 

       850        860        870        880        890        900 
DFEETSVSKH LGLYIDEMIK KLTALLKVKK VKTLTRSSQF LLLNLIEAFN KARALRDSLD 

       910        920        930        940        950        960 
LNGKRYCIML NQYVLSKHQR QLTSLPTREM AWAFHSSNKA VLLDHTKKLH GKPLLWNAVE 

       970        980        990       1000       1010       1020 
EYAIPFWLNE QLLKDVFLEL SRNHYAEIDD RNPENVSLYH IALHKINVFR DLWRLASWHK 

      1030       1040       1050       1060       1070       1080 
ESARTVKLLS NDFSKKKWKV VASKNAFALL SKHRYFYASA FFLLADSCYD AAKVCIRNLK 

      1090       1100       1110       1120       1130       1140 
SISLAIAMTR VYEGDDGPTL KRLINEYVLP LAVSQNDRWL TNWSFTILKQ EKKALQSLVS 

      1150       1160       1170       1180       1190       1200 
PIRSLVSDND FIQLYTSDKN DQEIHENKDS QRNSTNEPVR NKFPSDDPAF ILLYECLRSS 

      1210       1220       1230       1240       1250       1260 
NVQIDESLEY RFVLHNANVY CQLGCDLIAL GLLRNWKFQK NPATAHLETE DYMVTDKQSA 

      1270       1280       1290 
VEKTHDSPKL GKTLMGQDIR PTPDDVPEFN ESAFSFE 

References and documentation are available.

Number of amino acids: 1297

Molecular weight: 148739.36

Theoretical pI: 6.37

Amino acid composition: 
Ala (A)  74	  5.7%
Arg (R)  61	  4.7%
Asn (N)  80	  6.2%
Asp (D)  84	  6.5%
Cys (C)  26	  2.0%
Gln (Q)  54	  4.2%
Glu (E)  58	  4.5%
Gly (G)  44	  3.4%
His (H)  35	  2.7%
Ile (I)  78	  6.0%
Leu (L) 162	 12.5%
Lys (K)  70	  5.4%
Met (M)  16	  1.2%
Phe (F)  76	  5.9%
Pro (P)  48	  3.7%
Ser (S) 130	 10.0%
Thr (T)  63	  4.9%
Trp (W)  24	  1.9%
Tyr (Y)  50	  3.9%
Val (V)  64	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 142
Total number of positively charged residues (Arg + Lys): 131

Atomic composition:

Carbon      C	      6720
Hydrogen    H	     10350
Nitrogen    N	      1778
Oxygen      O	      1959
Sulfur      S	        42

Formula: C6720H10350N1778O1959S42
Total number of atoms: 20849

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   208125
Abs 0.1% (=1 g/l)   1.399, assuming all pairs of Cys residues form cystines


Ext. coefficient   206500
Abs 0.1% (=1 g/l)   1.388, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.00
This classifies the protein as unstable.



Aliphatic index: 92.18

Grand average of hydropathicity (GRAVY): -0.214