ProtParam
User-provided sequence:
10 20 30 40 50 60
MPLLDTFKSF IQSPTESKVK QATNEDETSG ATGTLMNEIS ILTYSPKTVR EIIQVIRKRL
70 80 90 100 110 120
LLGQNRRNSH RNCIQVMKTL TLVSYLMNNG SNEFIKWLKG NMILIEILED FQVQDPRDER
130 140 150 160 170 180
KAEDIQKLSR NVLGLLQDDG LLEKQRKDVI QFRSSISTPG RKSTDNSHLK LEEMRSELTR
190 200 210 220 230 240
QSLEKKAKPP TTSTSLDFQR QRTRNTHEYA RFSLDPLAEE DSEDTPGVAG GISKLSFRPK
SSNNPFR
References and
documentation are available.
Number of amino acids: 247
Molecular weight: 28271.09
Theoretical pI: 9.38
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1227
Hydrogen H 2013
Nitrogen N 361
Oxygen O 389
Sulfur S 7
Formula: C
1227H
2013N
361O
389S
7
Total number of atoms: 3997
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.353, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.353, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.48
This classifies the protein as unstable.
Aliphatic index: 79.72
Grand average of hydropathicity (GRAVY): -0.760