ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPLMSNSERD KLESTLRRRF FYTPSFEIYG GVSGLFDLGP PGCQLQNNLI RLWREHFIME 

        70         80         90        100        110        120 
ENMLQVDGPM LTPYDVLKTS GHVDKFTDWM CRNPKTGEYY RADHLIEQTL KKRLLDKDVN 

       130        140        150        160        170        180 
PQDMKNMEKI LTTIDGFSGP ELNLVMQEYN INDPVTNDVL DALTSFNLMF ETKIGASGQL 

       190        200        210        220        230        240 
KAFLRPETAQ GQFLNFNKLL EINQGKIPFA SASIGKSFRN EISPRSGLLR VREFLMAEIE 

       250        260        270        280        290        300 
HFVDPLNKSH AKFNEVLNEE IPLLSRRLQE SGEVQLPVKM TIGEAVNSGM VENETLGYFM 

       310        320        330        340        350        360 
ARVHQFLLNI GINKDKFRFR QHLKNEMAHY ATDCWDGEIL TSYGWIECVG CADRAAFDLT 

       370        380        390        400        410        420 
VHSKKTGRSL TVKQKLDTPK ERTEWVVEVN KKFFGSKFKQ KAKLIESVLS KFSQDELIRR 

       430        440        450        460        470        480 
HEELEKNGEF TCQVNGQIVK LDSSLVTIKM KTTLQHIREY IPNVIEPSFG LGRIIYCIFD 

       490        500        510        520        530        540 
HCFQVRVDSE SRGFFSFPLQ IAPIKVFVTT ISNNDGFPAI LKRISQALRK REIYFKIDDS 

       550        560        570        580        590        600 
NTSIGKKYAR NDELGTPFGI TIDFETIKDQ TVTLRERNSM RQVRGTITDV ISTIDKMLHN 

       610 
PDESDWDKST FGLSPVKI 

References and documentation are available.

Number of amino acids: 618

Molecular weight: 71018.45

Theoretical pI: 7.20

Amino acid composition: 
Ala (A)  21	  3.4%
Arg (R)  36	  5.8%
Asn (N)  34	  5.5%
Asp (D)  36	  5.8%
Cys (C)   8	  1.3%
Gln (Q)  25	  4.0%
Glu (E)  46	  7.4%
Gly (G)  37	  6.0%
His (H)  13	  2.1%
Ile (I)  44	  7.1%
Leu (L)  58	  9.4%
Lys (K)  46	  7.4%
Met (M)  18	  2.9%
Phe (F)  39	  6.3%
Pro (P)  25	  4.0%
Ser (S)  40	  6.5%
Thr (T)  38	  6.1%
Trp (W)   6	  1.0%
Tyr (Y)  13	  2.1%
Val (V)  35	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 82

Atomic composition:

Carbon      C	      3174
Hydrogen    H	      5007
Nitrogen    N	       863
Oxygen      O	       933
Sulfur      S	        26

Formula: C3174H5007N863O933S26
Total number of atoms: 10003

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52870
Abs 0.1% (=1 g/l)   0.744, assuming all pairs of Cys residues form cystines


Ext. coefficient    52370
Abs 0.1% (=1 g/l)   0.737, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.35
This classifies the protein as unstable.



Aliphatic index: 84.19

Grand average of hydropathicity (GRAVY): -0.397