ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPLPPSTLNQ KSNRVYSVAR VYKNACEERP QEYWDYEQGV TIDWGKISNY EIINKIGRGK 

        70         80         90        100        110        120 
YSEVFSGRCI VNNQKCVIKV LKPVKMKKIY RELKILTNLT GGPNVVGLYD IVQDADSKIP 

       130        140        150        160        170        180 
ALIFEEIKNV DFRTLYPTFK LPDIQYYFTQ LLIALDYCHS MGIMHRDVKP QNVMIDPTER 

       190        200        210        220        230        240 
KLRLIDWGLA EFYHPGVDYN VRVASRYHKG PELLVNLNQY DYSLDLWSVG CMLAAIVFKK 

       250        260        270        280        290        300 
EPFFKGSSNP DQLVKIATVL GTKELLGYLG KYGLHLPSEY DNIMRDFTKK SWTHFITSET 

       310        320        330 
KLAVPEVVDL IDNLLRYDHQ ERLTAKEAMD HKFFKTKFE 

References and documentation are available.

Number of amino acids: 339

Molecular weight: 39403.60

Theoretical pI: 8.68

Amino acid composition: 
Ala (A)  13	  3.8%
Arg (R)  15	  4.4%
Asn (N)  17	  5.0%
Asp (D)  21	  6.2%
Cys (C)   5	  1.5%
Gln (Q)  11	  3.2%
Glu (E)  20	  5.9%
Gly (G)  18	  5.3%
His (H)   8	  2.4%
Ile (I)  23	  6.8%
Leu (L)  34	 10.0%
Lys (K)  31	  9.1%
Met (M)   8	  2.4%
Phe (F)  14	  4.1%
Pro (P)  17	  5.0%
Ser (S)  16	  4.7%
Thr (T)  16	  4.7%
Trp (W)   5	  1.5%
Tyr (Y)  21	  6.2%
Val (V)  26	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1800
Hydrogen    H	      2798
Nitrogen    N	       464
Oxygen      O	       503
Sulfur      S	        13

Formula: C1800H2798N464O503S13
Total number of atoms: 5578

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59040
Abs 0.1% (=1 g/l)   1.498, assuming all pairs of Cys residues form cystines


Ext. coefficient    58790
Abs 0.1% (=1 g/l)   1.492, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.48
This classifies the protein as stable.



Aliphatic index: 91.65

Grand average of hydropathicity (GRAVY): -0.335