ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPLQLFGRDQ IVVHYDNGNM SNDDQNHQSV LGSWTRRAAA ALRTLMNKRI QRITLTHWLL 

        70         80         90        100        110        120 
LVIWVTSLWK FTSHYRQLYA NSAVFATLCT NILLFGISDI LAQSIACFYS YHVDPIPQIL 

       130        140        150        160        170        180 
NDTFHHVQNN RDVENGGGYE SDELSIFNDF TSEHSSYTDN DDYPELDRPL ATFKTDTFDF 

       190        200        210        220        230        240 
FRWGCFMFWG FFISFFQAPW YKFLNFFYTE DPTVVQVFER VLSDQLLYSP ISLYCFFMFS 

       250        260        270        280        290        300 
NYVMEGGDKD TLGKKIQRLY ISTLGCNYLV WPMVQFINFL IMPRDFQAPF SSSVGVVWNC 


FLSMRNASK 

References and documentation are available.

Number of amino acids: 309

Molecular weight: 36195.19

Theoretical pI: 5.56

Amino acid composition: 
Ala (A)  13	  4.2%
Arg (R)  14	  4.5%
Asn (N)  19	  6.1%
Asp (D)  21	  6.8%
Cys (C)   6	  1.9%
Gln (Q)  15	  4.9%
Glu (E)   8	  2.6%
Gly (G)  14	  4.5%
His (H)   8	  2.6%
Ile (I)  17	  5.5%
Leu (L)  30	  9.7%
Lys (K)   8	  2.6%
Met (M)   9	  2.9%
Phe (F)  30	  9.7%
Pro (P)  11	  3.6%
Ser (S)  25	  8.1%
Thr (T)  18	  5.8%
Trp (W)   9	  2.9%
Tyr (Y)  15	  4.9%
Val (V)  19	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1668
Hydrogen    H	      2446
Nitrogen    N	       418
Oxygen      O	       460
Sulfur      S	        15

Formula: C1668H2446N418O460S15
Total number of atoms: 5007

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72225
Abs 0.1% (=1 g/l)   1.995, assuming all pairs of Cys residues form cystines


Ext. coefficient    71850
Abs 0.1% (=1 g/l)   1.985, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.26
This classifies the protein as stable.



Aliphatic index: 81.36

Grand average of hydropathicity (GRAVY): -0.045