ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPLRNLTETH NFSSTNLDTD GTGDDHDGAP LSSSPSFGQQ NDNSTNDNAG LTNPFMGSDE 

        70         80         90        100        110        120 
ESNARDGESL SSSVHYQPQG SDSSLLHDNS RLDLSQNKGV SDYKGYYSRN NSRAVSTAND 

       130        140        150        160        170        180 
NSFLQPPHRA IASSPSLNSN LSKNDILSPP EFDRYPLVGS RVTSMTQLNH HGRSPTSSPG 

       190        200        210        220        230        240 
NESSASFSSN PFLGEQDFSP FGGYPASSFP LMIDEKEEDD YLHNPDPEEE ARLDRRRFID 

       250        260        270        280        290        300 
DFKYMDKRSA SGLAGVLLLF LAAIFIFIVL PALTFTGAID HESNTEEVTY LTQYQYPQLS 

       310        320        330        340        350        360 
AIRTSLVDPD TPDTAKTREA MDGSKWELVF SDEFNAEGRT FYDGDDPYWT APDVHYDATK 

       370        380        390        400        410        420 
DLEWYSPDAS TTVNGTLQLR MDAFKNHGLY YRSGMLQSWN KVCFTQGALE ISANLPNYGR 

       430        440        450        460        470        480 
VSGLWPGLWT MGNLGRPGYL ASTQGVWPYS YESCDAGITP NQSSPDGISY LPGQKLSICT 

       490        500        510        520        530        540 
CDGEDHPNQG VGRGAPEIDV LEGETDTKIG VGIASQSLQI APFDIWYMPD YDFIEVYNFT 

       550        560        570        580        590        600 
TTTMNTYAGG PFQQAVSAVS TLNVTWYEFG EYGGYFQKYA IEYLNDDDNG YIRWFVGDTP 

       610        620        630        640        650        660 
TYTIHAKALH PDGNIGWRRI SKEPMSIILN LGISNNWAYI DWQYIFFPVV MSIDYVRIYQ 

       670        680        690        700        710        720 
PSNAISVTCD PSDYPTYDYI QSHLNAFQNA NLTTWEDAGY TFPKNILTGK CTSSKFKLSS 

References and documentation are available.

Number of amino acids: 720

Molecular weight: 80122.85

Theoretical pI: 4.49

Amino acid composition: 
Ala (A)  42	  5.8%
Arg (R)  26	  3.6%
Asn (N)  46	  6.4%
Asp (D)  59	  8.2%
Cys (C)   6	  0.8%
Gln (Q)  27	  3.8%
Glu (E)  33	  4.6%
Gly (G)  55	  7.6%
His (H)  15	  2.1%
Ile (I)  34	  4.7%
Leu (L)  55	  7.6%
Lys (K)  20	  2.8%
Met (M)  13	  1.8%
Phe (F)  33	  4.6%
Pro (P)  46	  6.4%
Ser (S)  78	 10.8%
Thr (T)  50	  6.9%
Trp (W)  14	  1.9%
Tyr (Y)  41	  5.7%
Val (V)  27	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      3558
Hydrogen    H	      5289
Nitrogen    N	       935
Oxygen      O	      1147
Sulfur      S	        19

Formula: C3558H5289N935O1147S19
Total number of atoms: 10948

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   138465
Abs 0.1% (=1 g/l)   1.728, assuming all pairs of Cys residues form cystines


Ext. coefficient   138090
Abs 0.1% (=1 g/l)   1.723, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.82
This classifies the protein as unstable.



Aliphatic index: 64.92

Grand average of hydropathicity (GRAVY): -0.550