ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPNASQVYRS TRSSSPKTIS FEEAIIQGLA TDGGLFIPPT IPQVDQATLF NDWSKLSFQD 

        70         80         90        100        110        120 
LAFAIMRLYI AQEEIPDADL KDLIKRSYST FRSDEVTPLV QNVTGDKENL HILELFHGPT 

       130        140        150        160        170        180 
YAFKDVALQF VGNLFEYFLQ RTNANLPEGE KKQITVVGAT SGDTGSAAIY GLRGKKDVSV 

       190        200        210        220        230        240 
FILYPTGRIS PIQEEQMTTV PDENVQTLSV TGTFDNCQDI VKAIFGDKEF NSKHNVGAVN 

       250        260        270        280        290        300 
SINWARILAQ MTYYFYSFFQ ATNGKDSKKV KFVVPSGNFG DILAGYFAKK MGLPIEKLAI 

       310        320        330        340        350        360 
ATNENDILDR FLKSGLYERS DKVAATLSPA MDILISSNFE RLLWYLAREY LANGDDLKAG 

       370        380        390        400        410        420 
EIVNNWFQEL KTNGKFQVDK SIIEGASKDF TSERVSNEET SETIKKIYES SVNPKHYILD 

       430        440        450        460        470        480 
PHTAVGVCAT ERLIAKDNDK SIQYISLSTA HPAKFADAVN NALSGFSNYS FEKDVLPEEL 

       490        500        510 
KKLSTLKKKL KFIERADVEL VKNAIEEELA KMKL 

References and documentation are available.

Number of amino acids: 514

Molecular weight: 57474.25

Theoretical pI: 5.46

Amino acid composition: 
Ala (A)  41	  8.0%
Arg (R)  16	  3.1%
Asn (N)  29	  5.6%
Asp (D)  32	  6.2%
Cys (C)   2	  0.4%
Gln (Q)  19	  3.7%
Glu (E)  36	  7.0%
Gly (G)  28	  5.4%
His (H)   6	  1.2%
Ile (I)  36	  7.0%
Leu (L)  46	  8.9%
Lys (K)  43	  8.4%
Met (M)   7	  1.4%
Phe (F)  30	  5.8%
Pro (P)  19	  3.7%
Ser (S)  40	  7.8%
Thr (T)  31	  6.0%
Trp (W)   4	  0.8%
Tyr (Y)  18	  3.5%
Val (V)  31	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2591
Hydrogen    H	      4055
Nitrogen    N	       669
Oxygen      O	       788
Sulfur      S	         9

Formula: C2591H4055N669O788S9
Total number of atoms: 8112

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48945
Abs 0.1% (=1 g/l)   0.852, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   0.849, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.94
This classifies the protein as stable.



Aliphatic index: 87.68

Grand average of hydropathicity (GRAVY): -0.280