ProtParam
User-provided sequence:
10 20 30 40 50 60
MPNFQKPFSG SSDGNSVMND LGNKVAIKVF DCRSAQDGSE EQNVNVTTNQ MYLMFQSNNY
70 80 90 100 110 120
NVPPPNYNTE DLGSQGPPTH AYYAPFQHPI HLQPPVPPVY KNNTYSATDQ YSDSSFPNTS
130 140 150 160 170 180
GHTPVIDSNY YNDALASIPT TTTGSTTMTT DNGNTIDSEE YIDNMEVFSS EENENIDNVK
190 200 210 220 230 240
QTDLKSEKDS SLLSAASIVK KEQLSGFENF LPLSKTESPL VTADEIKSSL NLENIDNADS
250 260 270 280 290 300
MSFKLKTSPI RKHFHVKPKR ITRVRTGRVS HNIIEKKYRS NINDKIEQLR RTVPTLRVAY
310 320 330 340 350 360
KKCNDLPITS RDLADLDGLE PATKLNKASI LTKSIEYICH LERKCLQLSL ANQHLSNDTR
370 380 390 400 410 420
DSFVHLTEPS QPLSDNSSSE QVQKQTRSCQ RQRQRQPRQQ QPLHNIQYNI PHQNGLMSGT
430
NNSHDMDFNN AGDF
References and
documentation are available.
Number of amino acids: 434
Molecular weight: 48871.05
Theoretical pI: 6.02
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2109
Hydrogen H 3319
Nitrogen N 609
Oxygen O 701
Sulfur S 14
Formula: C
2109H
3319N
609O
701S
14
Total number of atoms: 6752
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22600
Abs 0.1% (=1 g/l) 0.462, assuming all pairs of Cys residues form cystines
Ext. coefficient 22350
Abs 0.1% (=1 g/l) 0.457, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.56
This classifies the protein as unstable.
Aliphatic index: 65.81
Grand average of hydropathicity (GRAVY): -0.855