ProtParam
User-provided sequence:
10 20 30 40 50 60
MPNGSIRNCA NAVADTVRQT FSRKTWEHKE QLQTITEQKK FFLRKVRWQI AILAHFGFAI
70 80 90 100 110 120
SFGIRSNFGV AKNRMVNNFT DAYGEVHERE FLWTGAEVGM MESSFFYGYA ASQIPAGVLA
130 140 150 160 170 180
AKFAPNKIFM LGILVASFMN ILSAISFNFH PYTDIFVMVV QAVQGLALGV LYPAMHGVWK
190 200 210 220 230 240
FWAPPLERSK LATTAFTGSS VGVMTGLPAS AYLVSHFSWS TPFYVFGVVG IIWSLIWMYV
250 260 270 280 290 300
SSHSPETHGY ISDDEKKQVT EKIGDVAVKN MSLTTLPWRD MMTSSAVWAI IICTFCRSWG
310 320 330 340 350 360
FFLLLGNQLT YMKDVLHIDI KNSGFISIFP QFGMCIVTLA TGQLCDYLRS SGKMSTEAVR
370 380 390 400 410 420
KSVNTFGFTV EAMMLGCLAF VRDPVIAVTC LVIACTGSGS VLSGFNVNHF DIAPRYAPIL
430 440 450 460 470 480
MGIANGLGAV AGVGGMVTNT VTYQNPDGWK WVFLLAMAID IFGVIFFLIF AKGDVLPWAR
490 500 510 520 530 540
EPEKEETFNE FVRRMSIKVR SLSRKTRNRE GDTSYEKMEE DSEMKPCSKK VEARAPAEKS
ESSS
References and
documentation are available.
Number of amino acids: 544
Molecular weight: 60543.28
Theoretical pI: 8.80
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2759
Hydrogen H 4239
Nitrogen N 709
Oxygen O 759
Sulfur S 33
Formula: C
2759H
4239N
709O
759S
33
Total number of atoms: 8499
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 98360
Abs 0.1% (=1 g/l) 1.625, assuming all pairs of Cys residues form cystines
Ext. coefficient 97860
Abs 0.1% (=1 g/l) 1.616, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.36
This classifies the protein as unstable.
Aliphatic index: 85.13
Grand average of hydropathicity (GRAVY): 0.188