ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPNITMGLKP DPVAPTNPTH HESNAAKEIR YRGVRKRPWG RYAAEIRDPV KKTRVWLGTF 

        70         80         90        100        110        120 
DTAQQAARAY DAAARDFRGV KAKTNFGVIV GSSPTQSSTV VDSPTAARFI TPPHLELSLG 

       130        140        150        160        170        180 
GGGACRRKIP LVHPVYYYNM ATYPKMTTCG VQSESETSSV VDFEGGAGKI SPPLDLDLNL 


APPAE 

References and documentation are available.

Number of amino acids: 185

Molecular weight: 20024.75

Theoretical pI: 9.42

Amino acid composition: 
Ala (A)  20	 10.8%
Arg (R)  13	  7.0%
Asn (N)   6	  3.2%
Asp (D)   9	  4.9%
Cys (C)   2	  1.1%
Gln (Q)   4	  2.2%
Glu (E)   8	  4.3%
Gly (G)  15	  8.1%
His (H)   4	  2.2%
Ile (I)   7	  3.8%
Leu (L)  10	  5.4%
Lys (K)  10	  5.4%
Met (M)   4	  2.2%
Phe (F)   5	  2.7%
Pro (P)  18	  9.7%
Ser (S)  12	  6.5%
Thr (T)  15	  8.1%
Trp (W)   2	  1.1%
Tyr (Y)   7	  3.8%
Val (V)  14	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       886
Hydrogen    H	      1398
Nitrogen    N	       254
Oxygen      O	       264
Sulfur      S	         6

Formula: C886H1398N254O264S6
Total number of atoms: 2808

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21555
Abs 0.1% (=1 g/l)   1.076, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   1.070, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.58
This classifies the protein as unstable.



Aliphatic index: 68.59

Grand average of hydropathicity (GRAVY): -0.431