ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPNSHGNVLN NISLNSKQNP RSISKSCPND KDARQKSFKT ISAQALVRVQ GAGYKLGDVK 

        70         80         90        100        110        120 
LKDAEVKEKN SLKKYDCKNA TQEKKEQEQV FEKTVAKGSV QKYITKTSKT NSLFIGNLKS 

       130        140        150        160        170        180 
TVTEEMLRKI FKRYQSFESA KVCRDFLTKK SLGYGYLNFK DKNDAESARK EFNYTVFFGQ 

       190        200        210        220        230        240 
EVKIMPSMKN TLFRKNIGTN VFFSNLPLEN PQLTTRSFYL IMIEYGNVLS CLLERRKNIG 

       250        260        270        280        290        300 
FVYFDNDISA RNVIKKYNNQ EFFGNKIICG LHFDKEVRTR PEFTKRKKMI GSDIVIEDEL 

       310        320        330        340        350        360 
LASNNLSDNA RSKTILVKNL PSDTTQEEVL DYFSTIGPIK SVFISEKQAN TPHKAFVTYK 

       370        380        390        400        410        420 
NEEESKKAQK CLNKTIFKNH TIWVGPGKDK PVHNQIGTNK KTKVYLKNLS FNCNKEFISQ 

       430        440        450        460        470        480 
LCLQEKIRFS EIKITNYNSL NWTFCGHVEC FSRSDAERLF NILDRRLIGS SLVEASWSKN 

       490        500        510        520        530        540 
NDNILNEIDY DDGNNNENYK KLINISSMMR FRTQELSAHQ KGLTSQFQQV VSPFSSYSNS 

       550        560        570        580        590        600 
YTNMNSLVAT PMKPHPAFNL ITNTVDEKLH QPKRTKQENA EILESLKKII NRNLQRISIS 

       610        620        630        640        650 
GLNKEENLRS ISEFIFDVFW EHDSERLSHF LLMTNTSLES QKILQKQVTR AAESLGFTV 

References and documentation are available.

Number of amino acids: 659

Molecular weight: 75919.58

Theoretical pI: 9.48

Amino acid composition: 
Ala (A)  24	  3.6%
Arg (R)  29	  4.4%
Asn (N)  63	  9.6%
Asp (D)  26	  3.9%
Cys (C)  10	  1.5%
Gln (Q)  29	  4.4%
Glu (E)  46	  7.0%
Gly (G)  25	  3.8%
His (H)  11	  1.7%
Ile (I)  44	  6.7%
Leu (L)  55	  8.3%
Lys (K)  73	 11.1%
Met (M)  11	  1.7%
Phe (F)  39	  5.9%
Pro (P)  17	  2.6%
Ser (S)  61	  9.3%
Thr (T)  39	  5.9%
Trp (W)   4	  0.6%
Tyr (Y)  19	  2.9%
Val (V)  34	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      3370
Hydrogen    H	      5363
Nitrogen    N	       937
Oxygen      O	      1015
Sulfur      S	        21

Formula: C3370H5363N937O1015S21
Total number of atoms: 10706

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50935
Abs 0.1% (=1 g/l)   0.671, assuming all pairs of Cys residues form cystines


Ext. coefficient    50310
Abs 0.1% (=1 g/l)   0.663, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.72
This classifies the protein as stable.



Aliphatic index: 77.19

Grand average of hydropathicity (GRAVY): -0.634