ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPNSNVRIPP TVPSKIIDVV DQALRARLLG GSTFNSGFDS LDSVLNLQFR LHYHVIGSNG 

        70         80         90        100        110        120 
PAKPVCDVLL KESQNLEKNM SMMEELNDYP EITKLVEKIL FNCLGILFFH RGQFQESQRC 

       130        140        150        160        170        180 
LLHSLKIHNN TASQKTALME QYDRYLIVEN LYYRGLVSQD INIMQNVFYK ELLAHVDTIP 

       190        200        210        220        230        240 
PESNGLLFEY ISLIVAKLRF NQIQDLAENF KTTVENPFIL FLYMIKKFQS PLKKHIDNDD 

       250        260        270        280        290        300 
LYLKFGQNVL LKAKFPTASE TNDEALEHFN VFLQYYFKFT HIKKIKVNPS WYNFIISSME 

       310        320        330        340        350        360 
KTFQSIEVSK TAMFLFQNLS DNSNDEIKKK TFKRESILNF VNFVKYNDKY YQLHDNSHRD 

       370        380        390        400        410        420 
IISFIDAYSF ILQNSSKTDS IENVFDYDNT VSTFATSLNS FYKEYNLPLM SQSESLDWLE 

       430        440        450        460        470        480 
NSTRCVYPGN ISKVLTNAWS TLYEIRKYQL DFLVSNNLTS YLCNAMMLST KEKDNADVEE 

       490        500        510        520        530        540 
QEEGEEEKAL RELQFKYSYT LAQQRHIETA IKTLESLILS KNPNYYKAWH LLALCRSVQE 

       550        560        570        580        590        600 
DKEMSYKIVC SVLEAMNESL QNNTLLLNDR WQFIHLKLTQ LALIEEIFGT LEALETLPEV 

       610        620        630        640        650        660 
FELYATLFPD SQPELNSMGP KYSQTKEYLL QMVWIFAANM YMRTKDNDED AKAAIKEASN 

       670        680        690        700        710        720 
VESKFKNLNC NIANGYLSII KDEPGVALKE FETVLYYDEN NLDALVGFAE LIFPEELGVE 

       730        740        750        760        770        780 
ETNLERYYTL SLDKKPGKRA KLTFVNDTDR SAAYARLKFL LECAILESIE AYYSPEVWWY 

       790        800        810 
LSLIYEKYQD DEYKNSLLKC IKYQELNPIR SLRYCNY 

References and documentation are available.

Number of amino acids: 817

Molecular weight: 95365.76

Theoretical pI: 5.24

Amino acid composition: 
Ala (A)  42	  5.1%
Arg (R)  24	  2.9%
Asn (N)  64	  7.8%
Asp (D)  41	  5.0%
Cys (C)  11	  1.3%
Gln (Q)  34	  4.2%
Glu (E)  69	  8.4%
Gly (G)  19	  2.3%
His (H)  14	  1.7%
Ile (I)  50	  6.1%
Leu (L) 103	 12.6%
Lys (K)  62	  7.6%
Met (M)  18	  2.2%
Phe (F)  45	  5.5%
Pro (P)  25	  3.1%
Ser (S)  63	  7.7%
Thr (T)  38	  4.7%
Trp (W)   8	  1.0%
Tyr (Y)  48	  5.9%
Val (V)  39	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 110
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      4326
Hydrogen    H	      6667
Nitrogen    N	      1085
Oxygen      O	      1285
Sulfur      S	        29

Formula: C4326H6667N1085O1285S29
Total number of atoms: 13392

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   116145
Abs 0.1% (=1 g/l)   1.218, assuming all pairs of Cys residues form cystines


Ext. coefficient   115520
Abs 0.1% (=1 g/l)   1.211, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.95
This classifies the protein as unstable.



Aliphatic index: 92.02

Grand average of hydropathicity (GRAVY): -0.335