ProtParam
User-provided sequence:
10 20 30 40 50 60
MPNVDEQRGK AATKRAESEQ SEEKGDRQEG DGDPKDESEE ATAKIKGRSR FTAALRSLSK
70 80 90 100 110 120
TKTKRERELD KSTDRETRSA EEEAQETPGE LEDVASSIST DSGALGKTKK KSLTRRLLLG
130 140 150 160 170 180
GLRKSGKTPK GRPHSLATSD DISVEHLETT PLPSPPGSPT SAGSSTLQIT ISGKKVEPSP
190 200 210 220 230 240
AKKSGKSRPK SDTDYLTPAA EERKPRKKTT TTTVTTRDTS SSTRTTKLLV SKKKPQSPER
250 260 270 280 290 300
ESPSASPSPL ITTTESTRDT PPRERAPAKP SRVQSASGTG AVRKSQSSSA SSRKLELMQQ
310 320 330 340 350 360
QQQRGHRLNA RPLAELANTE PQPSGQQSLD PSTSPPAAED GVSSAESAQT IANPPPVVRF
370 380 390 400 410 420
EVGSAVRSPL SAYEAALAAA ASQQPNSNEE LSDSSIRRLS FAQQQLILGD HDSIEGRRET
430 440 450 460 470 480
LHYQSVASEY SNQDSGSEDD SEEQADTTEP ENAKPMPAFG DLTMDQEMEP AIMTSTSDKR
490 500 510 520 530 540
EHLYKILVIG ELGTGKTSFI KRYVHQFFSQ NYRATIGVDF ALKVLQWDAN TIVRLQLWDI
550 560 570 580 590 600
AGQERFGNMT RVYYKEAVGA FIVFDVTRSG TFDCVSKWKE DLDSKVQLPD GSPIPCILLA
610 620 630 640 650 660
NKCDQEKQGI ITQPEKMDEY VRENGFAGWF ETSAKENINI DEAARALVNK ILINDKLISA
670 680
DLADGDKFNL SAADATGSDA KNKCSC
References and
documentation are available.
Number of amino acids: 686
Molecular weight: 74700.76
Theoretical pI: 5.94
Total number of negatively charged residues (Asp + Glu): 102
Total number of positively charged residues (Arg + Lys): 96
Atomic composition:
Carbon C 3195
Hydrogen H 5186
Nitrogen N 942
Oxygen O 1093
Sulfur S 13
Formula: C
3195H
5186N
942O
1093S
13
Total number of atoms: 10429
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37150
Abs 0.1% (=1 g/l) 0.497, assuming all pairs of Cys residues form cystines
Ext. coefficient 36900
Abs 0.1% (=1 g/l) 0.494, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.97
This classifies the protein as unstable.
Aliphatic index: 63.09
Grand average of hydropathicity (GRAVY): -0.841