ProtParam
User-provided sequence:
10 20 30 40 50 60
MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV
70 80 90 100 110 120
KLTIQNRQAQ IEVVPSASAL IIKALKEPPR DRKKQKNIKH NGNITFDEIV NIARQMRHRS
130 140 150 160
LARELSGTIK EILGTAQSVG CNVDGRHPHD IIDDINSGAV ECPAS
References and
documentation are available.
Number of amino acids: 165
Molecular weight: 17845.61
Theoretical pI: 9.48
Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 780
Hydrogen H 1299
Nitrogen N 233
Oxygen O 234
Sulfur S 5
Formula: C
780H
1299N
233O
234S
5
Total number of atoms: 2551
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7115
Abs 0.1% (=1 g/l) 0.399, assuming all pairs of Cys residues form cystines
Ext. coefficient 6990
Abs 0.1% (=1 g/l) 0.392, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.49
This classifies the protein as unstable.
Aliphatic index: 94.00
Grand average of hydropathicity (GRAVY): -0.363