ProtParam
User-provided sequence:
10 20 30 40 50 60
MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT KEFKGIKVTV
70 80 90 100 110 120
QLKIQNRQAA ASVVPSASSL VITALKEPPR DRKKDKNVKH SGNIQLDEII EIARQMRDKS
130 140 150 160
FGRTLASVTK EILGTAQSVG CRVDFKNPHD IIEGINAGEI EIPEN
References and
documentation are available.
Number of amino acids: 165
Molecular weight: 17822.62
Theoretical pI: 9.43
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 789
Hydrogen H 1310
Nitrogen N 224
Oxygen O 237
Sulfur S 3
Formula: C
789H
1310N
224O
237S
3
Total number of atoms: 2563
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.167, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.167, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.54
This classifies the protein as stable.
Aliphatic index: 94.00
Grand average of hydropathicity (GRAVY): -0.336