ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPPKKGKQAQ AAGKKKDNVD KTFGMKNKNR STKVQKYIKQ VQSQSDPKKE EMRLKKLEEK 

        70         80         90        100        110        120 
KRREAEEAER RALFNPVADQ RVRAGVDPKS MVCALFKLGN CNKGAKCKFS HDLNVGRRME 

       130        140        150        160        170        180 
KKDLYQDTRS EKENDTMDNW DEEKLRKVIL SKHGNPKTTT DKVCKYFIEA VENGKYGWFW 

       190        200        210        220        230        240 
ICPNGGDKCM YRHSLPEGFV LKTNEQKRLE RESLEKQPKI TLEEFIETER GKLDKSKLTP 

       250        260        270        280        290        300 
ITIANFAQWK KDHVIAKINA EKKLSSKRKP TGREIILKMS AENKSFETDN ADMPDDVTQG 

       310        320        330        340 
SAWDLTEFTD ALKKADHQDD GGIKDYGDGS NPTFDIKKAN SATLA 

References and documentation are available.

Number of amino acids: 345

Molecular weight: 39514.02

Theoretical pI: 9.38

Amino acid composition: 
Ala (A)  23	  6.7%
Arg (R)  18	  5.2%
Asn (N)  19	  5.5%
Asp (D)  27	  7.8%
Cys (C)   6	  1.7%
Gln (Q)  13	  3.8%
Glu (E)  29	  8.4%
Gly (G)  20	  5.8%
His (H)   5	  1.4%
Ile (I)  14	  4.1%
Leu (L)  21	  6.1%
Lys (K)  55	 15.9%
Met (M)   9	  2.6%
Phe (F)  12	  3.5%
Pro (P)  13	  3.8%
Ser (S)  17	  4.9%
Thr (T)  19	  5.5%
Trp (W)   5	  1.4%
Tyr (Y)   6	  1.7%
Val (V)  14	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      1723
Hydrogen    H	      2787
Nitrogen    N	       501
Oxygen      O	       532
Sulfur      S	        15

Formula: C1723H2787N501O532S15
Total number of atoms: 5558

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36815
Abs 0.1% (=1 g/l)   0.932, assuming all pairs of Cys residues form cystines


Ext. coefficient    36440
Abs 0.1% (=1 g/l)   0.922, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.40
This classifies the protein as stable.



Aliphatic index: 58.00

Grand average of hydropathicity (GRAVY): -1.098