ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPPKKQVEEK KVLLGRPGNN LKAGIVGLAN VGKSTFFQAI TRCPLGNPAN YPFATIDPEE 

        70         80         90        100        110        120 
ARVIVPSPRF DKLCEIYKKT ASEVPAHLTV YDIAGLTKGA SAGEGLGNAF LSHIRSVDSI 

       130        140        150        160        170        180 
YQVVRCFDDA EIIHVEGDVD PVRDLEIINQ ELRLKDIEFA QKALEGAEKI AKRGGQSLEV 

       190        200        210        220        230        240 
KQKKEEMDLI TKIIKLLESG QRVANHSWTS KEVEIINSMF LLTAKPCIYL INLSERDYIR 

       250        260        270        280        290        300 
KKNKHLLRIK EWVDKYSPGD LIIPFSVSLE ERLSHMSPED AEEELKKLQT ISALPKIITT 

       310        320        330        340        350        360 
MRQKLDLISF FTCGPDEVRE WTIRRGTKAP QAAGVIHNDL MNTFILAQVM KCEDVFEYKD 

       370        380        390 
DSAIKAAGKL MQKGKDYVVE DGDIIYFRAG AGKN 

References and documentation are available.

Number of amino acids: 394

Molecular weight: 44174.08

Theoretical pI: 7.05

Amino acid composition: 
Ala (A)  30	  7.6%
Arg (R)  19	  4.8%
Asn (N)  14	  3.6%
Asp (D)  24	  6.1%
Cys (C)   6	  1.5%
Gln (Q)  13	  3.3%
Glu (E)  33	  8.4%
Gly (G)  25	  6.3%
His (H)   7	  1.8%
Ile (I)  35	  8.9%
Leu (L)  35	  8.9%
Lys (K)  38	  9.6%
Met (M)   8	  2.0%
Phe (F)  14	  3.6%
Pro (P)  18	  4.6%
Ser (S)  21	  5.3%
Thr (T)  16	  4.1%
Trp (W)   3	  0.8%
Tyr (Y)  10	  2.5%
Val (V)  25	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      1975
Hydrogen    H	      3185
Nitrogen    N	       533
Oxygen      O	       583
Sulfur      S	        14

Formula: C1975H3185N533O583S14
Total number of atoms: 6290

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31775
Abs 0.1% (=1 g/l)   0.719, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.711, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.83
This classifies the protein as stable.



Aliphatic index: 95.30

Grand average of hydropathicity (GRAVY): -0.289