ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL 

        70         80         90        100        110        120 
NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE RYNRLLESED SGGAALSTGV 

       130        140        150        160        170        180 
TDLKAGDINP NAETQMARPD NGDLEDEEKE MLAEARARLL NTQGKKATRK IRERMLEESK 

       190        200        210        220        230        240 
RIAELQKRRE LKQAGINVAI KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK 

       250        260        270        280        290        300 
FEQFERKVNR KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS 

       310        320        330        340        350        360 
APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS NIKSGKQLKS 

       370        380        390        400        410        420 
RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK EFENERAMNE EDNFIEPPSQ 

       430        440        450        460        470        480 
NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA IDNKYNLLVA NAINKEPHMV PEDTVDFLKE 

       490        500        510        520        530        540 
VESRMQHITQ GRTSMKIQFK TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM 

       550        560        570        580        590 
CSTLCHHSLP ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI 

References and documentation are available.

Number of amino acids: 590

Molecular weight: 67730.80

Theoretical pI: 8.30

Amino acid composition: 
Ala (A)  42	  7.1%
Arg (R)  35	  5.9%
Asn (N)  32	  5.4%
Asp (D)  31	  5.3%
Cys (C)   3	  0.5%
Gln (Q)  36	  6.1%
Glu (E)  64	 10.8%
Gly (G)  23	  3.9%
His (H)   9	  1.5%
Ile (I)  29	  4.9%
Leu (L)  52	  8.8%
Lys (K)  63	 10.7%
Met (M)  15	  2.5%
Phe (F)  12	  2.0%
Pro (P)  31	  5.3%
Ser (S)  36	  6.1%
Thr (T)  28	  4.7%
Trp (W)   4	  0.7%
Tyr (Y)  18	  3.1%
Val (V)  27	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      2960
Hydrogen    H	      4790
Nitrogen    N	       848
Oxygen      O	       931
Sulfur      S	        18

Formula: C2960H4790N848O931S18
Total number of atoms: 9547

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48945
Abs 0.1% (=1 g/l)   0.723, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   0.721, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.79
This classifies the protein as unstable.



Aliphatic index: 73.93

Grand average of hydropathicity (GRAVY): -0.932