ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPQRIELTSE PVRKPRSTES SLLRKIQRAC RSTLPEPDLG LNLDVADYIN SKQGATPREA 

        70         80         90        100        110        120 
VLAIEKLVNN GDTQAAVFAL SLLDVLVKNC GYSIHLQISR KEFLNDLVKR FPEQPPLRYS 

       130        140        150        160        170        180 
KVQQMILEAI EEWYQTICKH ASYKDDLQYI NDMHKLLKYK GYTFPKVGSE NLAVLRPNDQ 

       190        200        210        220        230        240 
LRTPSELQEE QERAQAAKLE ELLRSGKPDD LKEANKLMKI MAGFKDDTKV AVKQAINNEL 

       250        260        270        280        290        300 
NKLKRKADLF NEMLTSADEP DLENEAIQEL YGDLKSAQPK FKKLIEEERD DDALVSNLSK 

       310        320        330        340        350        360 
FNDLVIQLLK RYKSIKGMKE EELNVPDTNE PAKELNLIDF DDDTTANTPS VTSPSKSLQP 

       370        380        390        400        410        420 
FDDLLGDFNK VSLSSPKSPQ ENDTVVDILG DAHSKSSGID LLDFDSQPGE SKTALSAYSN 

       430        440        450        460        470        480 
SIVLPNGLLN SSSNSKEITA QSQRHILNQS DHLRIDYELT RESMTKLRLV IFYSNISSDP 

       490        500        510        520        530        540 
ITNFALLVAS PKGTTLSLQP QSGNMLQSNS RDGIKQIASV EGISVNLGKP IKLKWKANYC 

       550 
TKGDSKEESG TTSLPTI 

References and documentation are available.

Number of amino acids: 557

Molecular weight: 62319.58

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  33	  5.9%
Arg (R)  22	  3.9%
Asn (N)  34	  6.1%
Asp (D)  41	  7.4%
Cys (C)   4	  0.7%
Gln (Q)  28	  5.0%
Glu (E)  41	  7.4%
Gly (G)  22	  3.9%
His (H)   6	  1.1%
Ile (I)  31	  5.6%
Leu (L)  70	 12.6%
Lys (K)  50	  9.0%
Met (M)   9	  1.6%
Phe (F)  14	  2.5%
Pro (P)  29	  5.2%
Ser (S)  55	  9.9%
Thr (T)  27	  4.8%
Trp (W)   2	  0.4%
Tyr (Y)  14	  2.5%
Val (V)  25	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2736
Hydrogen    H	      4435
Nitrogen    N	       749
Oxygen      O	       880
Sulfur      S	        13

Formula: C2736H4435N749O880S13
Total number of atoms: 8813

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32110
Abs 0.1% (=1 g/l)   0.515, assuming all pairs of Cys residues form cystines


Ext. coefficient    31860
Abs 0.1% (=1 g/l)   0.511, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.60
This classifies the protein as unstable.



Aliphatic index: 89.66

Grand average of hydropathicity (GRAVY): -0.573