ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPQSFTSIAR IGDYILKSPV LSKLCVPVAN QFINLAGYKK LGLKFDDLIA EENPIMQTAL 

        70         80         90        100        110        120 
RRLPEDESYA RAYRIIRAHQ TELTHHLLPR NEWIKAQEDV PYLLPYILEA EAAAKEKDEL 


DNIEVSK 

References and documentation are available.

Number of amino acids: 127

Molecular weight: 14564.79

Theoretical pI: 5.62

Amino acid composition: 
Ala (A)  13	 10.2%
Arg (R)   7	  5.5%
Asn (N)   5	  3.9%
Asp (D)   7	  5.5%
Cys (C)   1	  0.8%
Gln (Q)   5	  3.9%
Glu (E)  12	  9.4%
Gly (G)   3	  2.4%
His (H)   3	  2.4%
Ile (I)  11	  8.7%
Leu (L)  16	 12.6%
Lys (K)   9	  7.1%
Met (M)   2	  1.6%
Phe (F)   3	  2.4%
Pro (P)   8	  6.3%
Ser (S)   6	  4.7%
Thr (T)   4	  3.1%
Trp (W)   1	  0.8%
Tyr (Y)   6	  4.7%
Val (V)   5	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 16

Atomic composition:

Carbon      C	       658
Hydrogen    H	      1048
Nitrogen    N	       174
Oxygen      O	       192
Sulfur      S	         3

Formula: C658H1048N174O192S3
Total number of atoms: 2075

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.991, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.991, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.35
This classifies the protein as unstable.



Aliphatic index: 104.57

Grand average of hydropathicity (GRAVY): -0.304