ProtParam
User-provided sequence:
10 20 30 40 50 60
MPQTHEMSLN GTQYLKYELK DLESRAHDAK TPSTNEFYDD VESHGTEELV EAKLSFLNRI
70 80 90 100 110 120
AAGLSAETKG IEPITEDEKT DDSILNAASM WFSANMVLPA YAIGALGPMV FDLNFGQSVF
130 140 150 160 170 180
VIIFFNLLGL VSVAFFSVFG AELGLRQMIL SRYLVGNIAA RIFSFINFIA CIGWGIVNTV
190 200 210 220 230 240
ASSQVLNMVN PGHQCPLWAG CIVIIGATVI VTFFGYGVIH AYEKWAWVPN FAVFLVIIAR
250 260 270 280 290 300
LARSKKFVLG EWTSGPTTAG NVLSFGSTVY GFAAGWTTYA ADYTVYMPRK TNKYKIFFSL
310 320 330 340 350 360
VVGLATPLYF TMILGAAVAM AAIGDPAWKT YYDENSIGGL TFAVLVPNSV HGFGQFCCVL
370 380 390 400 410 420
LSLSTIANNV PNMYTIALSV QATWEPLAKV PRVIWTLLGN AAALGIAIPA CYYFSTFMNY
430 440 450 460 470 480
FMDSIGYYLA IYIAIACSEH FIYRRSFSAY NVDDWDSWER LPIGIAGTAA LIVGAFGVAL
490 500 510 520
GMCQTYWVGE ISRLIGDYGG DIGFELGLSW AFIVYNIARP FELKYFGR
References and
documentation are available.
Number of amino acids: 528
Molecular weight: 58050.16
Theoretical pI: 5.34
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 2702
Hydrogen H 4057
Nitrogen N 649
Oxygen O 732
Sulfur S 22
Formula: C
2702H
4057N
649O
732S
22
Total number of atoms: 8162
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 119220
Abs 0.1% (=1 g/l) 2.054, assuming all pairs of Cys residues form cystines
Ext. coefficient 118720
Abs 0.1% (=1 g/l) 2.045, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.96
This classifies the protein as stable.
Aliphatic index: 99.98
Grand average of hydropathicity (GRAVY): 0.478