ProtParam
User-provided sequence:
10 20 30 40 50 60
MPREFKSFGS TEKSLLSKGH GEPSYSEIYA EPENFLEIEV HNPKTHIPNG MDSKGMFTDY
70 80 90 100 110 120
EIICRTNLPS FHKRVSKVRR RYSDFEFFRK CLIKEISMLN HPKVMVPHLP GKILLSNRFS
130 140 150 160
NEVIEERRQG LNTWMQSVAG HPLLQSGSKV LVRFIEAEKF VG
References and
documentation are available.
Number of amino acids: 162
Molecular weight: 18769.65
Theoretical pI: 9.27
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 842
Hydrogen H 1320
Nitrogen N 234
Oxygen O 237
Sulfur S 8
Formula: C
842H
1320N
234O
237S
8
Total number of atoms: 2641
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.617, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.611, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.68
This classifies the protein as unstable.
Aliphatic index: 75.12
Grand average of hydropathicity (GRAVY): -0.500