ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPRETSLVTI IGEDSYKKLR SSRCLLVGAG GIGSELLKDI ILMEFGEIHI VDLDTIDLSN 

        70         80         90        100        110        120 
LNRQFLFRQK DIKQPKSTTA VKAVQHFNNS KLVPYQGNVM DISTFPLHWF EQFDIIFNAL 

       130        140        150        160        170        180 
DNLAARRYVN KISQFLSLPL IESGTAGFDG YMQPIIPGKT ECFECTKKET PKTFPVCTIR 

       190        200        210        220        230        240 
STPSQPIHCI VWAKNFLFNQ LFASETSGNE DDNNQDWGTD DAEEIKRIKQ ETNELYELQK 

       250        260        270        280        290        300 
IIISRDASRI PEILNKLFIQ DINKLLAIEN LWKTRTKPVP LSDSQINTPT KTAQSASNSV 

       310        320        330        340        350        360 
GTIQEQISNF INITQKLMDR YPKEQNHIEF DKDDADTLEF VATAANIRSH IFNIPMKSVF 

       370        380        390        400        410        420 
DIKQIAGNII PAIATTNAIV AGASSLISLR VLNLLKYAPT TKYTDLNMAF TAKASNLSQN 

       430        440        450        460        470        480 
RYLSNPKLAP PNKNCPVCSK VCRGVIKLSS DCLNKMKLSD FVVLIREKYS YPQDISLLDA 

       490        500        510        520        530        540 
SNQRLLFDYD FEDLNDRTLS EINLGNGSII LFSDEEGDTM IRKAIELFLD VDDELPCNTC 

       550        560        570        580        590        600 
SLPDVEVPLI KANNSPSKNE EEEKNEKGAD VVATTNSHGK DGIVILDDDE GEITIDAEPI 

       610        620        630 
NGSKKRPVDT EISEAPSNKR TKLVNEPTNS DIVELD 

References and documentation are available.

Number of amino acids: 636

Molecular weight: 71258.92

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  36	  5.7%
Arg (R)  23	  3.6%
Asn (N)  48	  7.5%
Asp (D)  48	  7.5%
Cys (C)  11	  1.7%
Gln (Q)  24	  3.8%
Glu (E)  43	  6.8%
Gly (G)  25	  3.9%
His (H)   7	  1.1%
Ile (I)  61	  9.6%
Leu (L)  59	  9.3%
Lys (K)  47	  7.4%
Met (M)   9	  1.4%
Phe (F)  27	  4.2%
Pro (P)  32	  5.0%
Ser (S)  50	  7.9%
Thr (T)  40	  6.3%
Trp (W)   4	  0.6%
Tyr (Y)  12	  1.9%
Val (V)  30	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3152
Hydrogen    H	      5038
Nitrogen    N	       842
Oxygen      O	       993
Sulfur      S	        20

Formula: C3152H5038N842O993S20
Total number of atoms: 10045

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40505
Abs 0.1% (=1 g/l)   0.568, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.560, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.24
This classifies the protein as unstable.



Aliphatic index: 92.92

Grand average of hydropathicity (GRAVY): -0.343