ProtParam
User-provided sequence:
10 20 30 40 50 60
MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS
70 80 90 100 110 120
RYIYTLYYKR KAISKDLYDW LIKEKYADKL LIAKWRKTGY EKLCCLRCIQ KNETNNGSTC
130 140 150
ICRVPRAQLE EEARKKGTQV SFHQCVHCGC RGCASTD
References and
documentation are available.
Number of amino acids: 157
Molecular weight: 18447.39
Theoretical pI: 9.54
Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 808
Hydrogen H 1306
Nitrogen N 240
Oxygen O 232
Sulfur S 11
Formula: C
808H
1306N
240O
232S
11
Total number of atoms: 2597
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27430
Abs 0.1% (=1 g/l) 1.487, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 1.460, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.99
This classifies the protein as stable.
Aliphatic index: 69.04
Grand average of hydropathicity (GRAVY): -0.852