ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPRLEEIDDF NDIDDLDMEL AELDPSLRTP IAPKITPKVV RSQDQENPAF LPGTNNNSNS 

        70         80         90        100        110        120 
NNNSSNEKEQ LSFINPKTGK VERSEAISKK DLEEVKRFQV LYPCYFDINR SHKEGRRVPK 

       130        140        150        160        170        180 
ELAVENPLAK TMADAVRELG ILCIFEGEKC HPQDFGNPGR IRVLFKENGQ LIGAATKFKG 

       190        200        210        220        230        240 
GKRQLMKAVG EYMKRHPTTI ESLREIPYGP DFDNIEFKKI PRVKGFKMNE IVPLHSPFLM 

       250        260        270 
GHPMTKSVYE TPKITAAEKS FKPPKNKYKV VRR 

References and documentation are available.

Number of amino acids: 273

Molecular weight: 31169.82

Theoretical pI: 9.14

Amino acid composition: 
Ala (A)  13	  4.8%
Arg (R)  17	  6.2%
Asn (N)  18	  6.6%
Asp (D)  14	  5.1%
Cys (C)   3	  1.1%
Gln (Q)   7	  2.6%
Glu (E)  25	  9.2%
Gly (G)  15	  5.5%
His (H)   5	  1.8%
Ile (I)  17	  6.2%
Leu (L)  19	  7.0%
Lys (K)  29	 10.6%
Met (M)   8	  2.9%
Phe (F)  14	  5.1%
Pro (P)  23	  8.4%
Ser (S)  14	  5.1%
Thr (T)  11	  4.0%
Trp (W)   0	  0.0%
Tyr (Y)   6	  2.2%
Val (V)  15	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1384
Hydrogen    H	      2214
Nitrogen    N	       388
Oxygen      O	       408
Sulfur      S	        11

Formula: C1384H2214N388O408S11
Total number of atoms: 4405

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9065
Abs 0.1% (=1 g/l)   0.291, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.287, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.74
This classifies the protein as unstable.



Aliphatic index: 72.12

Grand average of hydropathicity (GRAVY): -0.740