ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPRRPSSGRR VLKHQPLTFS PFMRLLSFAS MARRDLSHPE DCQCSDEDIS FDEKQKIPNL 

        70         80         90        100        110        120 
PRKGKEEQVS DSSDEEDSQE DVQADFEFFD PKPTDFHGVK ILLQNYLDDK EWDLSSFVDC 

       130        140        150        160        170        180 
ILEQTTVGTV VKVADDEDES VFALVTALNM ARDKDNKCFR ELKEFLRKVC SEKNIANNLE 

       190        200        210        220        230        240 
MLLEKKAQDV GLLVSQRVMN LPPQLLPPLY DGLFDEVSWA IEDEPTEKLR RSFRFKSYLL 

       250        260        270        280        290        300 
VTKIYKLKNP KQRKPRHGEE DIEDTVFLKP EDELFLELSS WSFTFPMRSQ LVTSQEMKNY 

       310        320 
QLMGLVMAVE ANKIPKFRQM LNSLID 

References and documentation are available.

Number of amino acids: 326

Molecular weight: 37946.28

Theoretical pI: 5.01

Amino acid composition: 
Ala (A)  12	  3.7%
Arg (R)  19	  5.8%
Asn (N)  12	  3.7%
Asp (D)  29	  8.9%
Cys (C)   5	  1.5%
Gln (Q)  16	  4.9%
Glu (E)  30	  9.2%
Gly (G)   8	  2.5%
His (H)   4	  1.2%
Ile (I)  10	  3.1%
Leu (L)  38	 11.7%
Lys (K)  27	  8.3%
Met (M)  11	  3.4%
Phe (F)  20	  6.1%
Pro (P)  19	  5.8%
Ser (S)  26	  8.0%
Thr (T)  11	  3.4%
Trp (W)   3	  0.9%
Tyr (Y)   5	  1.5%
Val (V)  21	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1684
Hydrogen    H	      2657
Nitrogen    N	       449
Oxygen      O	       515
Sulfur      S	        16

Formula: C1684H2657N449O515S16
Total number of atoms: 5321

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24200
Abs 0.1% (=1 g/l)   0.638, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.631, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.55
This classifies the protein as unstable.



Aliphatic index: 79.79

Grand average of hydropathicity (GRAVY): -0.585