ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPRRRLLKLL FIGKLRVSCK HYSTAGDPTK LVNLSEFTPD KIRNFGIVAH VDHGKSTLAD 

        70         80         90        100        110        120 
RLLEMCGAVP PGQKQMLDKL QVERERGITV KAQTAALRHR GYLLNLIDTP GHVDFSAEVS 

       130        140        150        160        170        180 
RSLAVCDGIL LLVAANQGVQ AQTIANFWLA FEKNIQIIPV INKIDLPGAD IKSVETQLKN 

       190        200        210        220        230        240 
LFEFNPEECL HISAKSGLNV DKVLEAIIDR VPAPTAIIDA PFRSMIFDSY FDHFRGAIAH 

       250        260        270        280        290        300 
IMVKEGSVKK GDKIQSYQNE KVYDVSEVGV MRPEMVKCTE LRAGQVGYLV CNMRTVNEAV 

       310        320        330        340        350        360 
VGETLFAETT SRDSVKTFAE IKGVKPTVYA GLFPVETSDY ESLKQAVERL CLNDPSVTVI 

       370        380        390        400        410        420 
PDSSKALGLG WRIGFLGVLH MEVFGARLSQ EYDASVILCQ PSVEYRAKIK DNETIRKKRY 

       430        440        450        460        470        480 
DGMEEIRILD PSKFPDESDV DSFLEPMVKV RMIVPNEMMG TVNGLCSECR GERGEITSID 

       490        500        510        520        530        540 
STRLVILWRL PLAEVAVDFF ERLKKLTSGY ASFDYEPDGW QDTRLVKLTI LINSKEVSEF 

       550        560        570        580        590        600 
SQIIPAAMAR DRAKILVQRL KREIPRQQFE VTIKACIGSS TKALSQIVIQ PMKRDFSQLL 

       610        620        630        640 
KGNFGGGGME RLNKKLSHQK KGKERMKMVG NVQIPKEAFL NVLKR 

References and documentation are available.

Number of amino acids: 645

Molecular weight: 72268.99

Theoretical pI: 8.93

Amino acid composition: 
Ala (A)  42	  6.5%
Arg (R)  41	  6.4%
Asn (N)  22	  3.4%
Asp (D)  35	  5.4%
Cys (C)  11	  1.7%
Gln (Q)  25	  3.9%
Glu (E)  45	  7.0%
Gly (G)  42	  6.5%
His (H)  10	  1.6%
Ile (I)  45	  7.0%
Leu (L)  61	  9.5%
Lys (K)  50	  7.8%
Met (M)  19	  2.9%
Phe (F)  27	  4.2%
Pro (P)  28	  4.3%
Ser (S)  41	  6.4%
Thr (T)  28	  4.3%
Trp (W)   4	  0.6%
Tyr (Y)  13	  2.0%
Val (V)  56	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      3217
Hydrogen    H	      5215
Nitrogen    N	       889
Oxygen      O	       935
Sulfur      S	        30

Formula: C3217H5215N889O935S30
Total number of atoms: 10286

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41995
Abs 0.1% (=1 g/l)   0.581, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   0.572, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.53
This classifies the protein as stable.



Aliphatic index: 95.78

Grand average of hydropathicity (GRAVY): -0.165