ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSDIMEQRG VSTPSHFHED IHITSERQFG FMKTDMMPEN QGGRDRLSSM PKSSWTSESY 

        70         80         90        100        110        120 
QLKPQSSFSG SHPSGSPNAR NTTNGSQWES SLFSSSMSDL FSRKLRLQGS DMLSTMSANT 

       130        140        150        160        170        180 
VVTHREEEPS ESLEEIEAQT IGNLLPDEDD LFAEVTGEVG RKSRANTGDE LDEFDLFSSV 

       190        200        210        220        230        240 
GGMELDGDIF SSVSHRNGER GGNNSVGELN RGEIPSRTLL VGNISSNVED YELKVLFEQF 

       250        260        270        280        290        300 
GDIQALHTAC KNRGFIMVSY CDIRAAQNAA RALQNKLLRG TKLDIRYSIS KENPSQKDTS 

       310        320        330        340        350        360 
KGALLVNNLD SSISNQELNR LVKSYGEVKE IRRTMHDNSQ IYIEFFDVRA AAAALGGLNG 

       370        380        390        400        410        420 
LEVAGKKLQL VPTYPEGTRY TSQCAANDTE GCLPKTSYSN TSSGHIGRHF PGMISSTSSD 

       430        440        450        460        470        480 
GGSMRVIHNS IGSPVNSFIE RHRSLSIPIG FPPSANGISA SKPVGLQEHG HHFDNSNMGI 

       490        500        510        520        530        540 
QSMPNLHPHS FSEYVDNFAN GSPYTSSAFS EMVSDGSKAN EGFMIHNVRG VEGFSGGGIG 

       550        560        570        580        590        600 
SPMHQSSRRP INLWSNSNTQ QQNPSSGMMW PNSPSHINSI PTQRPPVTVF SRAPPIMVNM 

       610        620        630        640        650        660 
ASSPVHHHIG SAPVLNSPFW DRRQAYVAES LESSGFHIGS HGSMGIPGSS PSHPMDIGSH 

       670        680        690        700        710        720 
KTFSVGGNRM DVNSQNAVLR SPQQLSHLFP GRSPMGSMPG SFDSPNERYR NLSHRRSESS 

       730        740        750        760        770        780 
SSNADKKLYE LDVDRILRGE DRRTTLMIKN IPNKYTSKML LSAIDEHCKG TYDFLYLPID 

       790        800        810        820        830        840 
FKNKCNVGYA FINLIEPEKI VPFFKAFNGK KWEKFNSEKV ATLTYARIQG KTALIAHFQN 

       850        860        870        880        890        900 
SSLMNEDKRC RPILFHTDGP NAGDQEPFPM GSNIRSRPGK PRSSSIDNYN SFSISSVSEN 

       910 
REETPNGTDP FLKEN 

References and documentation are available.

Number of amino acids: 915

Molecular weight: 100788.06

Theoretical pI: 6.49

Amino acid composition: 
Ala (A)  41	  4.5%
Arg (R)  53	  5.8%
Asn (N)  65	  7.1%
Asp (D)  43	  4.7%
Cys (C)   7	  0.8%
Gln (Q)  31	  3.4%
Glu (E)  56	  6.1%
Gly (G)  75	  8.2%
His (H)  32	  3.5%
Ile (I)  50	  5.5%
Leu (L)  59	  6.4%
Lys (K)  38	  4.2%
Met (M)  32	  3.5%
Phe (F)  42	  4.6%
Pro (P)  56	  6.1%
Ser (S) 131	 14.3%
Thr (T)  39	  4.3%
Trp (W)   6	  0.7%
Tyr (Y)  20	  2.2%
Val (V)  39	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      4361
Hydrogen    H	      6804
Nitrogen    N	      1278
Oxygen      O	      1400
Sulfur      S	        39

Formula: C4361H6804N1278O1400S39
Total number of atoms: 13882

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63175
Abs 0.1% (=1 g/l)   0.627, assuming all pairs of Cys residues form cystines


Ext. coefficient    62800
Abs 0.1% (=1 g/l)   0.623, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.14
This classifies the protein as unstable.



Aliphatic index: 63.30

Grand average of hydropathicity (GRAVY): -0.625