ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSESSVSIY KLDQLEYQYH YLTKSLQKFE PRYPKTAKLY NCIGKKNKKK IEKLLNSLEL 

        70         80         90        100        110        120 
KTLDKELDES YSKLLNNKIH YYETHLSKCI KEQIQKISKK NSSKVKDAQK NKSPSIDIEK 

       130        140        150        160        170        180 
MLATQLSLDD LALFMTRFRL IKILHQRIKQ KSKKIEGDTN NKTWLNNNDY SGYINDKTSK 

       190        200        210        220        230        240 
WNPSNIWNEV ITKLPSCEKL NALIGQSKIV QNLTESFDLS ICLIFGFDVS AMKAKKYGAR 

       250        260        270        280        290        300 
EKTANANQTH SNIDYDTDDG NEKNAIDSKS NAIGAQTQSN KETTSDNEDL LIKEYEGMLG 

       310        320        330        340        350        360 
SSGDEGEGGG YLNPNINYNE VTDEEPSEAS SDEDDSDERF SDSEENEPRR KKPKLHNLPE 

       370        380        390        400        410        420 
LMAGYYSGND TEEESDEDNK NVKGKKKKRD TAEDRTAREQ MSNEPKRKNR RGQRARRKIW 

       430        440        450        460        470        480 
EKKYGSQAKH VQRELEKEME DRKQRQIEYE ARVAKREAKA ASLEASRSRE REDRRTETNN 

       490        500        510 
KKEKESASTG EEHPSWIAKR LAEEKLQKAK FEGKKIKFD 

References and documentation are available.

Number of amino acids: 519

Molecular weight: 60054.05

Theoretical pI: 8.86

Amino acid composition: 
Ala (A)  29	  5.6%
Arg (R)  28	  5.4%
Asn (N)  39	  7.5%
Asp (D)  34	  6.6%
Cys (C)   4	  0.8%
Gln (Q)  21	  4.0%
Glu (E)  58	 11.2%
Gly (G)  22	  4.2%
His (H)   8	  1.5%
Ile (I)  29	  5.6%
Leu (L)  39	  7.5%
Lys (K)  73	 14.1%
Met (M)   8	  1.5%
Phe (F)   9	  1.7%
Pro (P)  13	  2.5%
Ser (S)  47	  9.1%
Thr (T)  24	  4.6%
Trp (W)   5	  1.0%
Tyr (Y)  20	  3.9%
Val (V)   9	  1.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      2595
Hydrogen    H	      4185
Nitrogen    N	       757
Oxygen      O	       855
Sulfur      S	        12

Formula: C2595H4185N757O855S12
Total number of atoms: 8404

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57550
Abs 0.1% (=1 g/l)   0.958, assuming all pairs of Cys residues form cystines


Ext. coefficient    57300
Abs 0.1% (=1 g/l)   0.954, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.14
This classifies the protein as unstable.



Aliphatic index: 61.71

Grand average of hydropathicity (GRAVY): -1.279