ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSEVTPKVP ERPSRRKTSE LFPLSGSESG DIKANSEPPT PAGTPNVPTR RPILKAKTMT 

        70         80         90        100        110        120 
SFESGMDQES LPKVPLQRPV RRSTTEELNN VMNNTSKELE EIESLISKHN IHNVSRKKSP 

       130        140        150        160        170        180 
TSVEEGKVAA IHQNGQRSAS DNKTSTNPSP LEKNEHEGAE GNESAISPSN LVNKSNNEVT 

       190        200        210        220        230        240 
EHSDSEDLTE KQKVHAALDN EAGDRSHFEE KLIPGDMKVQ VDVSKDVEEG SLNALPPSGI 

       250        260        270        280        290        300 
TESDDKAEKF TKHPESSLEE LQKHQEQQEE KIFQNPTDEE STTSLNEKQE GKDNMEVNSQ 

       310        320        330        340        350        360 
PQGPSDTETV IAATSSNVPS QIASEEENDV PVIPRSRPKK DFEAHVQKEE LPNTQEKRVS 

       370        380        390        400        410        420 
EECDSTLIST EEESKIPKIP SERPKRRAPP PVPKKPSSRI AAFQEMLQKQ QQQDLHNNGN 

       430        440        450        460        470        480 
SSATTASADI AKKHTDSSIT SDTTKADFTS KLNGLFALPG MVNPGQLPPS LEKKLSSPDT 

       490        500        510        520        530        540 
ESKLGPQDQS QAKTGPLGGT RRGRGPRGRK LPSKVASVEK IEEDDNTNKI EIFNNWNVSS 

       550        560        570        580        590        600 
SFSKEKVLID TTPGEQAERA LDEKSKSIPE EQREQSPNKM EAALCPFELD EKEKLPANAE 

       610        620        630        640        650        660 
SDPLSQLPQT NAVGNRKAIS EESLSPSEAI ANRDQNDTTE IQEQQMEDQM EVDMERELSG 

       670 
GYEDVDSALH SEEASFHSL 

References and documentation are available.

Number of amino acids: 679

Molecular weight: 74762.93

Theoretical pI: 4.91

Amino acid composition: 
Ala (A)  40	  5.9%
Arg (R)  28	  4.1%
Asn (N)  42	  6.2%
Asp (D)  37	  5.4%
Cys (C)   2	  0.3%
Gln (Q)  37	  5.4%
Glu (E)  87	 12.8%
Gly (G)  29	  4.3%
His (H)  14	  2.1%
Ile (I)  27	  4.0%
Leu (L)  42	  6.2%
Lys (K)  56	  8.2%
Met (M)  12	  1.8%
Phe (F)  13	  1.9%
Pro (P)  55	  8.1%
Ser (S)  82	 12.1%
Thr (T)  42	  6.2%
Trp (W)   1	  0.1%
Tyr (Y)   1	  0.1%
Val (V)  32	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 124
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      3168
Hydrogen    H	      5127
Nitrogen    N	       927
Oxygen      O	      1132
Sulfur      S	        14

Formula: C3168H5127N927O1132S14
Total number of atoms: 10368

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7115
Abs 0.1% (=1 g/l)   0.095, assuming all pairs of Cys residues form cystines


Ext. coefficient     6990
Abs 0.1% (=1 g/l)   0.093, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 68.24
This classifies the protein as unstable.



Aliphatic index: 59.19

Grand average of hydropathicity (GRAVY): -1.097