ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSHPNFIFR WIGLFSDKFR RQTTGIDENS NLQINGGDSS SSGSDETPVL SSVECYACTQ 

        70         80         90        100        110        120 
VGVPAFHSTS CDQAHAPEWR ASAGSSLVPI QEGSVPNPAR TRFRRLKGPF GEVLDPRSKR 

       130        140        150        160        170        180 
VQRWNRALLL ARGMALAVDP LFFYALSIGR TTGPACLYMD GAFAAVVTVL RTCLDAVHLW 

       190        200        210        220        230        240 
HVWLQFRLAY VSRESLVVGC GKLVWDPRAI ASHYARSLTG FWFDVIVILP VPQAVFWLVV 

       250        260        270        280        290        300 
PKLIREEKVK LIMTILLLIF LFQFLPKIYH CICLMRRMQK VTGYIFGTIW WGFALNLIAY 

       310        320        330        340        350        360 
FIASHVAGGC WYVLAIQRVA SCIRQQCMRT GNCNLSLACK EEVCYQFVSP TSTVGYPCLS 

       370        380        390        400        410        420 
GNLTSVVNKP MCLDSNGPFR YGIYRWALPV ISSNSLAVKI LYPIFWGLMT LSTFANDLEP 

       430        440        450        460        470        480 
TSNWLEVIFS IVMVLSGLLL FTLLIGNIQV FLHAVMAKKR KMQIRCRDME WWMKRRQLPS 

       490        500        510        520        530        540 
RLRQRVRRFE RQRWNALGGE DELELIHDLP PGLRRDIKRY LCFDLINKVP LFRGMDDLIL 

       550        560        570        580        590        600 
DNICDRAKPR VFSKDEKIIR EGDPVQRMIF IMRGRVKRIQ SLSKGVLATS TLEPGGYLGD 

       610        620        630        640        650        660 
ELLSWCLRRP FLDRLPPSSA TFVCLENIEA FSLGSEDLRY ITDHFRYKFA NERLKRTARY 

       670        680        690        700        710        720 
YSSNWRTWAA VNIQMAWRRR RKRTRGENIG GSMSPVSENS IEGNSERRLL QYAAMFMSIR 


PHDHLE 

References and documentation are available.

Number of amino acids: 726

Molecular weight: 83241.21

Theoretical pI: 9.68

Amino acid composition: 
Ala (A)  46	  6.3%
Arg (R)  68	  9.4%
Asn (N)  26	  3.6%
Asp (D)  29	  4.0%
Cys (C)  21	  2.9%
Gln (Q)  23	  3.2%
Glu (E)  31	  4.3%
Gly (G)  45	  6.2%
His (H)  13	  1.8%
Ile (I)  46	  6.3%
Leu (L)  81	 11.2%
Lys (K)  26	  3.6%
Met (M)  21	  2.9%
Phe (F)  38	  5.2%
Pro (P)  36	  5.0%
Ser (S)  55	  7.6%
Thr (T)  29	  4.0%
Trp (W)  21	  2.9%
Tyr (Y)  21	  2.9%
Val (V)  50	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3763
Hydrogen    H	      5900
Nitrogen    N	      1052
Oxygen      O	      1001
Sulfur      S	        42

Formula: C3763H5900N1052O1001S42
Total number of atoms: 11758

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   148040
Abs 0.1% (=1 g/l)   1.778, assuming all pairs of Cys residues form cystines


Ext. coefficient   146790
Abs 0.1% (=1 g/l)   1.763, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.78
This classifies the protein as unstable.



Aliphatic index: 94.53

Grand average of hydropathicity (GRAVY): -0.014