ProtParam
User-provided sequence:
10 20 30 40 50 60
MPSHRNSNLK FCTVCASNNN RSMESHKVLQ EAGYNVSSYG TGSAVRLPGL SIDKPNVYSF
70 80 90 100 110 120
GTPYNDIYND LLSQSADRYK SNGLLQMLDR NRRLKKAPEK WQESTKVFDF VFTCEERCFD
130 140 150 160 170 180
AVCEDLMNRG GKLNKIVHVI NVDIKDDDEN AKIGSKAILE LADMLNDKIE QCEKDDIPFE
190 200
DCIMDILTEW QSSHSQLPSL YAPSYY
References and
documentation are available.
Number of amino acids: 206
Molecular weight: 23469.39
Theoretical pI: 5.15
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1022
Hydrogen H 1597
Nitrogen N 281
Oxygen O 327
Sulfur S 13
Formula: C
1022H
1597N
281O
327S
13
Total number of atoms: 3240
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24785
Abs 0.1% (=1 g/l) 1.056, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 1.040, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.64
This classifies the protein as unstable.
Aliphatic index: 75.24
Grand average of hydropathicity (GRAVY): -0.584