ProtParam
User-provided sequence:
10 20 30 40 50 60
MPSIDCKRLQ KLAPKSPENQ SKCISRLRYM VMLDQVESDE GGNSSTRPYV WAVLLNAPPR
70 80 90 100 110 120
NADEYIRYVR QGPSPMAQKI QNDVSRTLVV ESQFHSRVSQ SSLSRLLNAY VWKRGALYVQ
130 140 150 160 170 180
GMNVLASPFL YACKSENQAF QFFDRLLQNE CPLYVLPNID GVHRGAKLLD KCLEVLDHRL
190 200 210 220 230 240
YTYLLSKGLT AKIYALPSIL TLSACTAPLS EALTIWDFLF AYGIHLNILC VIAQMFIFRE
250 260 270 280 290
QLIDHPSPMT LLRTFPPLNA KNIMKITILL ISKLPPELYN LLARHAWDSE AGVLIDRLT
References and
documentation are available.
Number of amino acids: 299
Molecular weight: 33988.71
Theoretical pI: 9.00
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1538
Hydrogen H 2443
Nitrogen N 411
Oxygen O 426
Sulfur S 15
Formula: C
1538H
2443N
411O
426S
15
Total number of atoms: 4833
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41745
Abs 0.1% (=1 g/l) 1.228, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 1.217, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.93
This classifies the protein as unstable.
Aliphatic index: 106.02
Grand average of hydropathicity (GRAVY): 0.005