ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSRNDGPAL LKRRSAKPFA GNLRLRRLTK AYLEPLDDNN KEMSPTVAKI DQIYDLTQEL 

        70         80         90        100        110        120 
IVQCQSVTSG LFPRYSKDRD VGYVKDSIYC AMACWACSVA YKRLDDDRGR QTELRQTAVK 

       130        140        150        160        170        180 
TMRGILFSWM QQSKNLDAFK SQNSSEFALH ARFDLSSGMG LETPNDDLHY GHLQMDLVAL 

       190        200        210        220        230        240 
YLLTLVQMIS AGCKVIYTHH EILLVKIKTI FQFFNKNGFE IFSNKKRKNS FSNKKKYIFK 

       250 
TNFNFRNESL HFPIFQFF 

References and documentation are available.

Number of amino acids: 258

Molecular weight: 29948.53

Theoretical pI: 9.53

Amino acid composition: 
Ala (A)  15	  5.8%
Arg (R)  16	  6.2%
Asn (N)  15	  5.8%
Asp (D)  16	  6.2%
Cys (C)   5	  1.9%
Gln (Q)  13	  5.0%
Glu (E)   9	  3.5%
Gly (G)  11	  4.3%
His (H)   6	  2.3%
Ile (I)  13	  5.0%
Leu (L)  27	 10.5%
Lys (K)  22	  8.5%
Met (M)   8	  3.1%
Phe (F)  19	  7.4%
Pro (P)   8	  3.1%
Ser (S)  20	  7.8%
Thr (T)  12	  4.7%
Trp (W)   2	  0.8%
Tyr (Y)  10	  3.9%
Val (V)  11	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1346
Hydrogen    H	      2102
Nitrogen    N	       370
Oxygen      O	       379
Sulfur      S	        13

Formula: C1346H2102N370O379S13
Total number of atoms: 4210

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26150
Abs 0.1% (=1 g/l)   0.873, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.865, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.53
This classifies the protein as unstable.



Aliphatic index: 78.64

Grand average of hydropathicity (GRAVY): -0.402