ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK RSEPNADSNN 

        70         80         90        100        110        120 
MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF LCLVVTMGSS LYVSSVPELV 

       130        140        150        160        170        180 
ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM 

       190        200        210        220        230        240 
RIILPLRFLS GVFASPALSV GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT 

       250        260        270        280        290        300 
EAKGWRWSEW IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT 

       310        320        330        340        350        360 
TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM SMGISGLPFI 

       370        380        390        400        410        420 
GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK RTTPYRGARD AETGELLPVV 

       430        440        450        460        470        480 
PEKFLIACKF GSVALPIGLF WQAWTARSDV HWMAPVAAGV PFGFGLILIF FSVLMYFSTC 

       490        500        510        520        530        540 
YPPLTVASCL AANNLLRYVM SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK 

       550        560        570        580        590        600 
WGSKLRHKSQ FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER 

       610        620        630        640        650 
LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM ATDASARMV 

References and documentation are available.

Number of amino acids: 659

Molecular weight: 73222.87

Theoretical pI: 8.35

Amino acid composition: 
Ala (A)  47	  7.1%
Arg (R)  31	  4.7%
Asn (N)  20	  3.0%
Asp (D)  27	  4.1%
Cys (C)   5	  0.8%
Gln (Q)  13	  2.0%
Glu (E)  33	  5.0%
Gly (G)  46	  7.0%
His (H)   9	  1.4%
Ile (I)  38	  5.8%
Leu (L)  66	 10.0%
Lys (K)  32	  4.9%
Met (M)  24	  3.6%
Phe (F)  39	  5.9%
Pro (P)  43	  6.5%
Ser (S)  56	  8.5%
Thr (T)  47	  7.1%
Trp (W)  13	  2.0%
Tyr (Y)  22	  3.3%
Val (V)  48	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      3345
Hydrogen    H	      5190
Nitrogen    N	       848
Oxygen      O	       938
Sulfur      S	        29

Formula: C3345H5190N848O938S29
Total number of atoms: 10350

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   104530
Abs 0.1% (=1 g/l)   1.428, assuming all pairs of Cys residues form cystines


Ext. coefficient   104280
Abs 0.1% (=1 g/l)   1.424, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.80
This classifies the protein as unstable.



Aliphatic index: 89.80

Grand average of hydropathicity (GRAVY): 0.078