ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSTTLLFPQ KHIRAIPGKI YAFFRELVSG VIISKPDLSH HYSCENATKE EGKDAADEEK 

        70         80         90        100        110        120 
TTTSLFPESN NIDRSLNGGC SVIPCSMDVS DLNTPISITL SPENRIKSEV NAKSLLGSRP 

       130        140        150        160        170        180 
EQDTGAPIKM STGVTSSPLS PSGSTPEHST KVLNNGEEEF ICHYCDATFR IRGYLTRHIK 

       190        200        210        220        230        240 
KHAIEKAYHC PFFNSATPPD LRCHNSGGFS RRDTYKTHLK ARHVLYPKGV KPQDRNKSSG 

       250        260        270        280        290        300 
HCAQCGEYFS TIENFVENHI ESGDCKALPQ GYTKKNEKRS GKLRKIKTSN GHSRFISTSQ 

       310        320        330        340        350        360 
SVVEPKVLFN KDAVEAMTIV ANNSSGNDII SKYGNNKLML NSENFKVDIP KRKRKYIKKK 

       370        380        390        400        410        420 
QQQVSGSTVT TPEVATQNNQ EVAPDEISSA TIFSPFDTHL LEPVPSSSSE SSAEVMFHGK 

       430        440        450        460        470        480 
QMKNFLIDIN SFTNQQQQAQ DNPSFLPLDI EQSSYDLSED AMSYPIISTQ SNRDCTQYDN 

       490        500        510 
TKISQILQSQ LNPEYLSENH MRETQQYLNF YNDNFGSQF 

References and documentation are available.

Number of amino acids: 519

Molecular weight: 58087.88

Theoretical pI: 7.25

Amino acid composition: 
Ala (A)  23	  4.4%
Arg (R)  20	  3.9%
Asn (N)  37	  7.1%
Asp (D)  25	  4.8%
Cys (C)  11	  2.1%
Gln (Q)  26	  5.0%
Glu (E)  34	  6.6%
Gly (G)  25	  4.8%
His (H)  17	  3.3%
Ile (I)  33	  6.4%
Leu (L)  31	  6.0%
Lys (K)  39	  7.5%
Met (M)   9	  1.7%
Phe (F)  23	  4.4%
Pro (P)  30	  5.8%
Ser (S)  63	 12.1%
Thr (T)  34	  6.6%
Trp (W)   0	  0.0%
Tyr (Y)  17	  3.3%
Val (V)  22	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2530
Hydrogen    H	      3973
Nitrogen    N	       715
Oxygen      O	       815
Sulfur      S	        20

Formula: C2530H3973N715O815S20
Total number of atoms: 8053

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25955
Abs 0.1% (=1 g/l)   0.447, assuming all pairs of Cys residues form cystines


Ext. coefficient    25330
Abs 0.1% (=1 g/l)   0.436, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.15
This classifies the protein as unstable.



Aliphatic index: 64.82

Grand average of hydropathicity (GRAVY): -0.710