ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPSVCHTSPI EKIIQQGHRI QNDSLIPSKR TKLAHTELTA HYATEDSHVE KHFLHNGSNF 

        70         80         90        100        110        120 
DGIDNVRYQN QPSPLTFITP NNTVDSSDWV PQFSSMKIDD SLEFSSEYKR LYSNYESQQR 

       130        140        150        160        170        180 
LNSSRQHLPF KNCMIRKTSC TYPPQKTLRQ QRQGNRDNPT DAFQFDAEFQ VLEREIQKER 

       190        200        210        220        230        240 
YEPITRRDEK WFDQDQSELQ RIATDIVKCC TPPPSSASSS STLSSSVESK LSESKFIQLM 

       250        260        270        280 
RNISSGDVTL KKNADGNSAS ELFSSNNGEL VGNRHIFVKD EIHKDILD 

References and documentation are available.

Number of amino acids: 288

Molecular weight: 33049.60

Theoretical pI: 6.21

Amino acid composition: 
Ala (A)   9	  3.1%
Arg (R)  17	  5.9%
Asn (N)  18	  6.2%
Asp (D)  20	  6.9%
Cys (C)   5	  1.7%
Gln (Q)  20	  6.9%
Glu (E)  19	  6.6%
Gly (G)   8	  2.8%
His (H)  10	  3.5%
Ile (I)  18	  6.2%
Leu (L)  19	  6.6%
Lys (K)  18	  6.2%
Met (M)   4	  1.4%
Phe (F)  13	  4.5%
Pro (P)  15	  5.2%
Ser (S)  38	 13.2%
Thr (T)  17	  5.9%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.4%
Val (V)  11	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1431
Hydrogen    H	      2243
Nitrogen    N	       417
Oxygen      O	       467
Sulfur      S	         9

Formula: C1431H2243N417O467S9
Total number of atoms: 4567

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21680
Abs 0.1% (=1 g/l)   0.656, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.648, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 63.17
This classifies the protein as unstable.



Aliphatic index: 64.31

Grand average of hydropathicity (GRAVY): -0.891