ProtParam
User-provided sequence:
10 20 30 40 50 60
MPTILYNTNS SLITKYRRPN ASNQYKGFLS KKGHTRLNSK SSGDIWEKDC SHTKNSGNDV
70 80 90 100 110 120
SFESEFEKDS VEYLRDLCFS IYPNSLHQKI RSIEALPDLQ VNTFIALIFQ NFVKSWYGIK
130 140 150 160 170 180
IPTDDSKFLT ELYNLVQDLI TYLKSSKINY HALLLDYIPC LLSSHLKALN DSSQNNDLVY
190 200 210 220 230 240
EQYCKLTLYD SKRYPMLFTE IIQSKMSTKS LLQRSFLDSF LNELVFGHIF NSIAEPYYLL
250 260 270 280 290 300
EGLNKICIRI KLNSAGNTRN EVTHGKPKCD PWLFVSNVKH KILQMTRLLA YSTSTEAANM
310 320 330 340 350 360
NTAEIQETAF LQRYIFTFFT DDFFKLSMRK PFLFSICRTL QHWISKLNAL NRVMYRTFDN
370 380 390 400 410 420
IVQTKITSPV TIGNLFSLLR HSLFPNDNMM GPPRVLPVGD AFLEFREECI SNLWDVCMTY
430 440 450 460
KLDHILAIKR SDIADLIICI SKNRDCNKLL IYRIIDCVIA QLP
References and
documentation are available.
Number of amino acids: 463
Molecular weight: 53730.12
Theoretical pI: 8.81
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2433
Hydrogen H 3804
Nitrogen N 634
Oxygen O 693
Sulfur S 22
Formula: C
2433H
3804N
634O
693S
22
Total number of atoms: 7586
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59540
Abs 0.1% (=1 g/l) 1.108, assuming all pairs of Cys residues form cystines
Ext. coefficient 58790
Abs 0.1% (=1 g/l) 1.094, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.42
This classifies the protein as stable.
Aliphatic index: 96.03
Grand average of hydropathicity (GRAVY): -0.170