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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPTILYNTNS SLITKYRRPN ASNQYKGFLS KKGHTRLNSK SSGDIWEKDC SHTKNSGNDV

70 80 90 100 110 120
SFESEFEKDS VEYLRDLCFS IYPNSLHQKI RSIEALPDLQ VNTFIALIFQ NFVKSWYGIK

130 140 150 160 170 180
IPTDDSKFLT ELYNLVQDLI TYLKSSKINY HALLLDYIPC LLSSHLKALN DSSQNNDLVY

190 200 210 220 230 240
EQYCKLTLYD SKRYPMLFTE IIQSKMSTKS LLQRSFLDSF LNELVFGHIF NSIAEPYYLL

250 260 270 280 290 300
EGLNKICIRI KLNSAGNTRN EVTHGKPKCD PWLFVSNVKH KILQMTRLLA YSTSTEAANM

310 320 330 340 350 360
NTAEIQETAF LQRYIFTFFT DDFFKLSMRK PFLFSICRTL QHWISKLNAL NRVMYRTFDN

370 380 390 400 410 420
IVQTKITSPV TIGNLFSLLR HSLFPNDNMM GPPRVLPVGD AFLEFREECI SNLWDVCMTY

430 440 450 460
KLDHILAIKR SDIADLIICI SKNRDCNKLL IYRIIDCVIA QLP


References and documentation are available.

Number of amino acids: 463 Molecular weight: 53730.12 Theoretical pI: 8.81
Amino acid composition: 
Ala (A) 17 3.7% Arg (R) 21 4.5% Asn (N) 33 7.1% Asp (D) 26 5.6% Cys (C) 12 2.6% Gln (Q) 15 3.2% Glu (E) 19 4.1% Gly (G) 12 2.6% His (H) 11 2.4% Ile (I) 39 8.4% Leu (L) 58 12.5% Lys (K) 33 7.1% Met (M) 10 2.2% Phe (F) 27 5.8% Pro (P) 17 3.7% Ser (S) 43 9.3% Thr (T) 27 5.8% Trp (W) 5 1.1% Tyr (Y) 21 4.5% Val (V) 17 3.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 45 Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 2433 Hydrogen H 3804 Nitrogen N 634 Oxygen O 693 Sulfur S 22 Formula: C2433H3804N634O693S22 Total number of atoms: 7586 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 59540 Abs 0.1% (=1 g/l) 1.108, assuming all pairs of Cys residues form cystines Ext. coefficient 58790 Abs 0.1% (=1 g/l) 1.094, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 39.42 This classifies the protein as stable. Aliphatic index: 96.03 Grand average of hydropathicity (GRAVY): -0.170