ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPTLVNGPRR DSTEGFDTDI ITLPRFIIEH QKQFKNATGD FTLVLNALQF AFKFVSHTIR 

        70         80         90        100        110        120 
RAELVNLVGL AGASNFTGDQ QKKLDVLGDE IFINAMRASG IIKVLVSEEQ EDLIVFPTNT 

       130        140        150        160        170        180 
GSYAVCCDPI DGSSNLDAGV SVGTIASIFR LLPDSSGTIN DVLRCGKEMV AACYAMYGSS 

       190        200        210        220        230        240 
THLVLTLGDG VDGFTLDTNL GEFILTHPNL RIPPQKAIYS INEGNTLYWN ETIRTFIEKV 

       250        260        270        280        290        300 
KQPQADNNNK PFSARYVGSM VADVHRTFLY GGLFAYPCDK KSPNGKLRLL YEAFPMAFLM 

       310        320        330        340 
EQAGGKAVND RGERILDLVP SHIHDKSSIW LGSSGEIDKF LDHIGKSQ 

References and documentation are available.

Number of amino acids: 348

Molecular weight: 38262.58

Theoretical pI: 5.64

Amino acid composition: 
Ala (A)  23	  6.6%
Arg (R)  15	  4.3%
Asn (N)  19	  5.5%
Asp (D)  24	  6.9%
Cys (C)   5	  1.4%
Gln (Q)  11	  3.2%
Glu (E)  16	  4.6%
Gly (G)  30	  8.6%
His (H)   8	  2.3%
Ile (I)  25	  7.2%
Leu (L)  34	  9.8%
Lys (K)  18	  5.2%
Met (M)   7	  2.0%
Phe (F)  20	  5.7%
Pro (P)  15	  4.3%
Ser (S)  24	  6.9%
Thr (T)  21	  6.0%
Trp (W)   2	  0.6%
Tyr (Y)   9	  2.6%
Val (V)  22	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1710
Hydrogen    H	      2681
Nitrogen    N	       459
Oxygen      O	       513
Sulfur      S	        12

Formula: C1710H2681N459O513S12
Total number of atoms: 5375

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   0.644, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.638, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.65
This classifies the protein as stable.



Aliphatic index: 91.06

Grand average of hydropathicity (GRAVY): -0.099