ProtParam
User-provided sequence:
10 20 30 40 50 60
MPTNNTPFLT IFLLFLGLLR FDSFPGLEAA TGKLASIPGL YVFGDSLVDA GNNNYLPISI
70 80 90 100 110 120
SKANYPHNGV DFPNKKPTGR FCNGKNAADA IAEKFGLPLP PPYLSLRGLL KREKRKSAAV
130 140 150 160 170 180
TGVNFASGGA GIFNSSDQKL GQAIPLSKQV NNWLSIHEEV MKLEPSAAQL HLSKSLFTVV
190 200 210 220 230 240
IGSNDLFDYF GSFKLRRQSN PQQYTQLMAD KLKEQLKRIH DSGARRFLII GVAQIGCTPG
250 260 270 280 290 300
KRAKNSTLHE CDEGANMWCS LYNEALVKML QQLKQELQGS ITYTYFDNYK SLHDIISNPA
310 320 330 340 350 360
RYGFADVTSA CCGNGELNAD LPCLPLAKLC SDRTKHLFWD RYGHPTEAAA RTIVDLMLTD
370
DTHYSSPITL TQLVST
References and
documentation are available.
Number of amino acids: 376
Molecular weight: 41447.41
Theoretical pI: 8.77
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1857
Hydrogen H 2908
Nitrogen N 504
Oxygen O 544
Sulfur S 14
Formula: C
1857H
2908N
504O
544S
14
Total number of atoms: 5827
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36370
Abs 0.1% (=1 g/l) 0.877, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.865, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.41
This classifies the protein as stable.
Aliphatic index: 86.20
Grand average of hydropathicity (GRAVY): -0.223