ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS VDSDREFLNS 

        70         80         90        100        110        120 
KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFTETL VNWKTGHCQR NWLLNKSFCC 

       130        140        150        160        170        180 
NGVVNEVTST SNLLLKRQEF ECEAQGLWIA WKGHVSPFII FMLLSVLFAL ISTLLVKYVA 

       190        200        210        220        230        240 
PMATGSGISE IKVWVSGFEY NKEFLGFLTL VIKSVALPLA ISSGLSVGKE GPSVHYATCC 

       250        260        270        280        290        300 
GYLLTKWLLR DTLTYSSQYE YITAASGAGV AVAFGAPIGG VLFGLEEIAS ANRFNSSTLW 

       310        320        330        340        350        360 
KSYYVALVAI TTLKYIDPFR NGRVILFNVT YDRDWKVQEI PIFIALGIFG GLYGKYISKW 

       370        380        390        400        410        420 
NINFIHFRKM YLSSWPVQEV LFLATLTALI SYFNEFLKLD MTESMGILFH ECVKNDNTST 

       430        440        450        460        470        480 
FSHRLCQLDE NTHAFEFLKI FTSLCFATVI RALLVVVSYG ARVPAGIFVP SMAVGATFGR 

       490        500        510        520        530        540 
AVSLLVERFI SGPSVITPGA YAFLGAAATL SGITNLTLTV VVIMFELTGA FMYIIPLMIV 

       550        560        570        580        590        600 
VAITRIILST SGISGGIADQ MIMVNGFPYL EDEQDEEEEE TLEKYTAEQL MSSKLITINE 

       610        620        630        640        650        660 
TIYLSELESL LYDSASEYSV HGFPITKDED KFEKEKRCIG YVLKRHLASK IMMQSVNSTK 

       670        680        690        700        710        720 
AQTTLVYFNK SNEELGHREN CIGFKDIMNE SPISVKKAVP VTLLFRMFKE LGCKTIIVEE 

       730        740        750        760        770 
SGILKGLVTA KDILRFKRIK YREVHGAKFT YNEALDRRCW SVIHFIIKRF TTNRNGNVI 

References and documentation are available.

Number of amino acids: 779

Molecular weight: 87591.67

Theoretical pI: 6.70

Amino acid composition: 
Ala (A)  49	  6.3%
Arg (R)  31	  4.0%
Asn (N)  35	  4.5%
Asp (D)  24	  3.1%
Cys (C)  14	  1.8%
Gln (Q)  16	  2.1%
Glu (E)  52	  6.7%
Gly (G)  50	  6.4%
His (H)  13	  1.7%
Ile (I)  68	  8.7%
Leu (L)  78	 10.0%
Lys (K)  43	  5.5%
Met (M)  17	  2.2%
Phe (F)  50	  6.4%
Pro (P)  22	  2.8%
Ser (S)  58	  7.4%
Thr (T)  57	  7.3%
Trp (W)  12	  1.5%
Tyr (Y)  30	  3.9%
Val (V)  60	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      4012
Hydrogen    H	      6250
Nitrogen    N	      1004
Oxygen      O	      1128
Sulfur      S	        31

Formula: C4012H6250N1004O1128S31
Total number of atoms: 12425

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   111575
Abs 0.1% (=1 g/l)   1.274, assuming all pairs of Cys residues form cystines


Ext. coefficient   110700
Abs 0.1% (=1 g/l)   1.264, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.24
This classifies the protein as stable.



Aliphatic index: 101.72

Grand average of hydropathicity (GRAVY): 0.212