ProtParam
User-provided sequence:
10 20 30 40 50 60
MPVAIITGAS SGIGKGTALL FAKKKYQLSL TGRNTDSLKE VAALCISEGA ISADDILITA
70 80 90 100 110 120
VELSSDEAPK AIVDATVQKF GRIDSLINSA GILRAGPVLD SGIEVYDELM NVNVRSLIRL
130 140 150 160 170 180
TRAALPHIIT TKGTVVNVSS INGPCPFAGV TYYCMSKSAV DQFTKCLALE MAPNGVRVNA
190 200 210 220 230 240
VCPGVIVTNI HRASGQDEAT YAAFLEKSKT THALGRPGTT SEVAEAILFL SSEKSSFTTG
250
QLLKVDGGRG IMHPR
References and
documentation are available.
Number of amino acids: 255
Molecular weight: 26768.86
Theoretical pI: 8.25
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1177
Hydrogen H 1934
Nitrogen N 324
Oxygen O 364
Sulfur S 10
Formula: C
1177H
1934N
324O
364S
10
Total number of atoms: 3809
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7700
Abs 0.1% (=1 g/l) 0.288, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.278, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.58
This classifies the protein as stable.
Aliphatic index: 100.63
Grand average of hydropathicity (GRAVY): 0.186