ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MPVEEPLATL SSIPDSSADQ APPLIADEFT LDLPRIPSLE LPLNVSTKHS SIQKAIKMCG 

        70         80         90        100        110        120 
GIEKVKEAFK EHGPIESQHG LQLYLNDDTD SDGSKSYFNE HPVIGKRVPF RDESVILKVT 

       130        140        150        160        170        180 
MPKGTLSKNN NSVKDSIKSL KDSNKLRVTP VSIVDNTIKF REMSDFQIKL DNVPSAREFK 

       190        200        210        220        230        240 
SSFGSLEWNN FKSFVNSVPD NDSQPQENIG NLILDRSVKI PSTDFQLPPP PKLSMVGFPL 

       250        260        270        280        290        300 
LYKYKANPFA KKKKNGVTEV KGTYIKNYQL FVHDLSDKTV IPSQAHEQVL YDFEVAKKTK 

       310        320        330        340        350        360 
VYPGTKSDSK FYESLEECLK ILRELFARRP IWVKRHLDGI VPKKIHHTMK IALALISYRF 

       370        380        390        400        410        420 
TMGPWRNTYI KFGIDPRSSV EYAQYQTEYF KIERKLLSSP IVKKNVPKPP PLVFESDTPG 

       430        440        450        460        470        480 
GIDSRFKFDG KRIPWYLMLQ IDLLIGEPNI AEVFHNVEYL DKANELTGWF KELDLVKIRR 

       490        500        510        520        530        540 
IVKYELGCMV QGNYEYNKYK LKYFKTMLFV KESMVPENKN SEEGMGVNTN KDADGDINMD 

       550        560        570        580        590        600 
AGSQMSSNAI EEDKGIAAGD DFDDNGAITE EPDDAALENE EMDTDQNLKV PASIDDDVDD 

       610        620        630        640 
VDADEEEQES FDVKTASFQD IINKIAKLDP KTAETMKSEL KGFVDEVDL 

References and documentation are available.

Number of amino acids: 649

Molecular weight: 73553.58

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)  30	  4.6%
Arg (R)  19	  2.9%
Asn (N)  35	  5.4%
Asp (D)  55	  8.5%
Cys (C)   3	  0.5%
Gln (Q)  19	  2.9%
Glu (E)  50	  7.7%
Gly (G)  31	  4.8%
His (H)  10	  1.5%
Ile (I)  43	  6.6%
Leu (L)  52	  8.0%
Lys (K)  67	 10.3%
Met (M)  16	  2.5%
Phe (F)  31	  4.8%
Pro (P)  41	  6.3%
Ser (S)  51	  7.9%
Thr (T)  27	  4.2%
Trp (W)   5	  0.8%
Tyr (Y)  21	  3.2%
Val (V)  43	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 105
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      3296
Hydrogen    H	      5174
Nitrogen    N	       852
Oxygen      O	      1013
Sulfur      S	        19

Formula: C3296H5174N852O1013S19
Total number of atoms: 10354

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58915
Abs 0.1% (=1 g/l)   0.801, assuming all pairs of Cys residues form cystines


Ext. coefficient    58790
Abs 0.1% (=1 g/l)   0.799, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.94
This classifies the protein as stable.



Aliphatic index: 80.92

Grand average of hydropathicity (GRAVY): -0.546