ProtParam
User-provided sequence:
10 20 30 40 50 60
MPVKILMPAL SPTMKEGTLA KWLVSEGEKI EAGQVIAEIE TDKATMEFEA VDEGVLGKIL
70 80 90 100 110 120
IPAKTAGVKV NQPIAVLLDD GEGEKELKKF LSTIDKPTVT DNKAETSDGD KIKNNPSSLP
130 140 150 160 170 180
ADKQQGRVIA TPLARKIASI NGIDLSLIGS GSGPDGRIVK NDLLKLLDDA PQVQMHGHCT
190 200 210 220 230 240
ETSIPISPMR RVIAQRLVES KQNVPHFYLS VTCYLQHLLS AKKKFYDCLE TKVTVNDFVI
250 260 270 280 290 300
KACAFALDKN PAMNVSWEGE FIRQNQTIDI SVAVAIPDGL ITPIVFSADK LSLSSISDEV
310 320 330 340 350 360
RELVDKAKAG RLQPREFQGG SFTVSNLGMY GIDEFTAIIN PPQAAILAVG AARKVPTVSA
370 380 390 400
DAVVVSDVVT LTLSCDHRVI DGALAARFMQ SLKKAIEDPV IML
References and
documentation are available.
Number of amino acids: 403
Molecular weight: 43468.34
Theoretical pI: 5.57
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1931
Hydrogen H 3162
Nitrogen N 514
Oxygen O 588
Sulfur S 15
Formula: C
1931H
3162N
514O
588S
15
Total number of atoms: 6210
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17210
Abs 0.1% (=1 g/l) 0.396, assuming all pairs of Cys residues form cystines
Ext. coefficient 16960
Abs 0.1% (=1 g/l) 0.390, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.89
This classifies the protein as stable.
Aliphatic index: 103.08
Grand average of hydropathicity (GRAVY): 0.020